92557-80-7
中文名称
5-羧基荧光素琥珀酰亚胺酯
英文名称
5-Carboxyfluorescein N-succinimidyl ester
CAS
92557-80-7
分子式
C25H15NO9
MDL 编号
MFCD00077322
分子量
473.39
MOL 文件
92557-80-7.mol
更新日期
2024/04/21 08:59:31
92557-80-7 结构式
基本信息
中文别名
5-羧基荧光素琥珀酰亚胺酯 英文别名
5(6)-CARBOXYFLUORESCEIN-NHS5(6)-FAM-NHS
5-CARBOXYFLUORESCEIN-NHS
5-CARBOXYFLUORESCEIN-N-HYDROXYSUCCINIMIDE ESTER
5-CARBOXYFLUORESCEIN N-SUCCINIMIDYL ESTER
5-CARBOXYFLUORESCEIN SE
5-CARBOXYFLUORESCEIN SUCCINIMIDE ESTER
5-CARBOXYFLUORESCEIN, SUCCINIMIDYL ESTER
5-FAM-NHS
5-FAM, SE
1-[[36Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9[9H]xanthen]-5-yl)carbonyl]oxy]-2,5-pyrrolidinedione
carboxyfluorescein-NHS ester
所属类别
生物化工：荧光成像物理化学性质
熔点>300°C (dec.)
沸点778.7±70.0 °C(Predicted)
密度1.74±0.1 g/cm3(Predicted)
储存条件−20°C
溶解度碱性溶液（微溶）、DMSO（微溶）、甲醇（微溶）
酸度系数(pKa)9.31±0.20(Predicted)
形态固体
颜色黄色到橙色
BRN7865828
InChIKeyGECIDMICWWDIBO-UHFFFAOYSA-N
常见问题列表
生物活性
5-FAM SE 是一种单一异构体，是一种用于标记肽，蛋白质和核苷酸的荧光标记试剂。5-FAM SE 可以与胺反应，并可以产生稳定的胺结合物。体外研究
The capillary electrophoresis (CE)-laser-induced fluorescent (LIF) method with 5-FAM SE (5-Carboxyfluorescein N-succinimidyl ester) derivatization for the analysis of serotonin can be used to quantify luminally released 5-HT with high sensitivity. CFSE is an excellent dye for the derivatization of 5-HT.
5-FAM SE (5-Carboxyfluorescein succinimidyl ester) is chosen as the labeling reagent to precapillary derivatize the two marker aminohydroxyphenylalanine (AHP) isomers produced after reductive hydrolysis of pheomelanin with hydriodic acid (HI).