TRANSCRIPTOR REVERSE TRANSCRIPTASE Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
Transcriptor Reverse Transcriptase is designed to transcribe RNA (mRNA, total RNA, viral RNA, and
in vitro-transcribed RNA) from a variety of sources, using conventional thermal cyclers and real-time PCR instruments (
e.g., the LightCycler
? Instruments) for the following applications:
- Synthesis of first-strand cDNA for use in subsequent amplification reactions
- RT-PCR of GC-rich RNA templates
- Cy3, Cy5, DIG, biotin, and aminoallyl labeling during cDNA synthesis
- Retrieving and cloning the 5′ and 3′ termini of mRNA by RACE
- Generation of cDNA libraries with large inserts
- Dideoxy DNA sequencing
- RNA sequencing
- 3′-end labeling of DNA fragments
- Generation of single-stranded probes for genomic footprints
- In the reverse transcription of RNA from human Papillomavirus E6, cortical and striatal tissues, muscle biopsies of Becker muscular dystrophy samples and miRNA stem-loop from oocytes and human cerebral microvascular endothelial cells (hCMVECs)
Allgemeine Beschreibung
In retroviruses like the human immunodeficiency virus type 1 (HIV-1), reverse transcriptase (RT) is the core enzyme. HIV-1 RT is made of two subunits of 66?kDa and 51?kDa (p66 and p5l). A human endogenous retrovirus of the HERV-K family codes for reverse transcriptase (RT) enzyme.
Biochem/physiol Actions
Human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) is essential for the catalytic conversion of single-stranded viral RNA into the double-stranded linear DNA that is integrated into host cell chromosomes.
TRANSCRIPTOR REVERSE TRANSCRIPTASE Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
