Duolink In Situ Wash Buffers, Fluorescence

CAS-Nr.

Englisch Name:: Duolink In Situ Wash Buffers, Fluorescence

Synonyma:: Duolink? In Situ Wash Buffers, Fluorescence

CBNumber:: CB02839145

Summenformel:

Molgewicht:: 0

MOL-Datei:: Mol file

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Eigenschaften Sicherheit Verwendungswort Produktionsfirma 2

Duolink In Situ Wash Buffers, Fluorescence Eigenschaften

storage temp.: 20-25°C

Sicherheit

Risiko- und Sicherheitserklärung
Gefahreninformationscode (GHS)

WGK Germany	3

Bildanzeige (GHS)

Alarmwort

Warnung

Gefahrenhinweise

Code	Gefahrenhinweise	Gefahrenklasse	Abteilung	Alarmwort	Symbol	P-Code
H315	Verursacht Hautreizungen.	Hautreizung	Kategorie 2	Warnung	src="/GHS07.jpg" width="20" height="20" />	P264, P280, P302+P352, P321,P332+P313, P362
H319	Verursacht schwere Augenreizung.	Schwere Augenreizung	Kategorie 2	Warnung	src="/GHS07.jpg" width="20" height="20" />	P264, P280, P305+P351+P338,P337+P313P
H335	Kann die Atemwege reizen.	Spezifische Zielorgan-Toxizität (einmalige Exposition)	Kategorie 3 (Atemwegsreizung)	Warnung	src="/GHS07.jpg" width="20" height="20" />

Sicherheit

P305+P351+P338

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Duolink In Situ Wash Buffers, Fluorescence Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

Duolink^?proximity ligation assay(PLA^?) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Use the Duolink^? In Situ Fluorescence Protocol for this product. A set of short instructionsis also available.

Visit our Duolink^? PLA Resource Center for information on how to run a Duolink^? experiment, applications, troubleshooting, and more.

To perform a complete Duolink^? PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink^? PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.

Specificity
Duolink^? In Situ fluorescence applications use two wash buffers. Wash Buffer A is used after the PLA Probe incubation step and Wash Buffer B is used after incubation with the amplification reagents. See datasheet for more information.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^?PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Linkage
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Duolink In Situ Wash Buffers, Fluorescence Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte

Duolink In Situ Wash Buffers, Fluorescence Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Global( 2)Lieferanten

Firmenname	Telefon	E-Mail	Land	Produktkatalog	Edge Rate
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80

Duolink? In Situ Wash Buffers, Fluorescence