Duolink In Situ Wash Buffers, Fluorescence
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Duolink In Situ Wash Buffers, Fluorescence 속성
- 저장 조건
- 20-25°C
안전
- 위험 및 안전 성명
- 위험 및 사전주의 사항 (GHS)
| WGK 독일 | 3 |
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| 그림문자(GHS): |
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| 신호 어: | Warning | ||||||||||||||||||||||||||||
| 유해·위험 문구: |
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| 예방조치문구: |
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Duolink In Situ Wash Buffers, Fluorescence C화학적 특성, 용도, 생산
용도
Duolink?proximity ligation assay(PLA?) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.Use the Duolink? In Situ Fluorescence Protocol for this product. A set of short instructionsis also available.
Visit our Duolink? PLA Resource Center for information on how to run a Duolink? experiment, applications, troubleshooting, and more.
To perform a complete Duolink? PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink? PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
Duolink? In Situ fluorescence applications use two wash buffers. Wash Buffer A is used after the PLA Probe incubation step and Wash Buffer B is used after incubation with the amplification reagents. See datasheet for more information.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink?PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Linkage
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Duolink In Situ Wash Buffers, Fluorescence 준비 용품 및 원자재
원자재
준비 용품
Duolink In Situ Wash Buffers, Fluorescence 공급 업체
글로벌( 2)공급 업체
| 공급자 | 전화 | 이메일 | 국가 | 제품 수 | 이점 |
|---|---|---|---|---|---|
| Sigma-Aldrich | 021-61415566 800-8193336 |
orderCN@merckgroup.com | China | 51471 | 80 |