3,3μ,5,5μ-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA

3,3μ,5,5μ-Tetramethylbenzidine  (TMB)  Liquid  Substrate  System  for  ELISA Struktur
CAS-Nr.
Englisch Name:
3,3μ,5,5μ-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA
Synonyma:
3,3μ,5,5μ-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA
CBNumber:
CB12091748
Summenformel:
Molgewicht:
0
MOL-Datei:
Mol file

3,3μ,5,5μ-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA Eigenschaften

storage temp. 
2-8°C

Sicherheit

3,3μ,5,5μ-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

TMB substrate is used in ELISA procedures, which utilize horseradish peroxidase conjugates. TMB substrate develops a soluble blue reaction product that can be read at 370 or 655 nm at 370 or 655 nm. The reaction is stopped with acid, forming a yellow reaction product which enables accurate intensity measurement at 450 nm.

How to use our TMB substrate:
  • Our TMB substrate is supplied as a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer. Prior to reaction with peroxidase, the substrate should be a colorless to light bluish-green solution. The substrate system develops a blue reaction product when reacted with peroxidase.
  • Upon reaching your assay endpoint with addition of acid, measure the TMB substrate intensity at 450 nm.
  • Our TMB Substrate is recommended for ELISA (microwell) procedures, and has been used in various assays, as a substrate for horseradish peroxidase antibody conjugate for detecting hendra virus glycoprotein, tetracosactide, sIgA and neutrophil extracellular traps (NETs).
  • 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System is not recommended for membrane applications. Since TMB substrate produces a soluble reaction product, it is not recommended for histochemistry or blotting.
Note:TMB substrate is light-sensitive and should be protected from direct sunlight or UV sources.

Allgemeine Beschreibung

TMB substrate is a chromogenic substrate for horseradish peroxidase - HRP.

3,3μ,5,5μ-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte


3,3μ,5,5μ-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Global( 7)Lieferanten
Firmenname Telefon E-Mail Land Produktkatalog Edge Rate
Sigma-Aldrich 021-61415566 800-8193336
orderCN@merckgroup.com China 51471 80

()Verwandte Suche:


  • 3,3μ,5,5μ-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA
  • Peroxidase SubstratesPlant Immunochemistry
  • Alphabetical ListingSubstrates, Buffers&Blockers
  • Bioassay
  • Detection Substrates
  • ELISA SubstratesELISA Substrates
  • Enzyme Substrates
  • Liquid Substrate SystemsEnzyme Substrates
  • Peroxidase
  • Substrates
  • Substrates by Enzyme
  • Substrates for Peroxidase
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