KAPA2G Fast HotStart Genotyping Mix

KAPA2G Fast HotStart Genotyping Mix Struktur
CAS-Nr.
Englisch Name:
KAPA2G Fast HotStart Genotyping Mix
Synonyma:
KAPA2G Fast HotStart Genotyping Mix
CBNumber:
CB14670103
Summenformel:
Molgewicht:
0
MOL-Datei:
Mol file

KAPA2G Fast HotStart Genotyping Mix Eigenschaften

Sicherheit
  • Risiko- und Sicherheitserklärung
  • Gefahreninformationscode (GHS)
Bildanzeige (GHS) GHS hazard pictograms
Alarmwort Warnung
Gefahrenhinweise
Code Gefahrenhinweise Gefahrenklasse Abteilung Alarmwort Symbol P-Code
H371 Kann die Organe schädigen. Spezifische Zielorgan-Toxizität Kategorie 2 Warnung P260, P264, P270, P309+P311, P405,P501
Sicherheit
P260 Dampf/Aerosol/Nebel nicht einatmen.
P264 Nach Gebrauch gründlich waschen.
P264 Nach Gebrauch gründlich waschen.
P270 Bei Gebrauch nicht essen, trinken oder rauchen.
P405 Unter Verschluss aufbewahren.
P501 Inhalt/Behälter ... (Entsorgungsvorschriften vom Hersteller anzugeben) zuführen.

KAPA2G Fast HotStart Genotyping Mix Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

KAPA2G Fast HotStart? Genotyping Mix has been used in:
  • Genomic DNA extraction and in the preparation of master mix for PCR
  • Extraction and amplification from mouse tail, ear, and toe tissue
  • Extraction and amplification of DNA from other animal tissues

Allgemeine Beschreibung

KAPA2G Fast HotStart? Mouse Genotyping Kits include KAPA Express Extract, a novel thermostable protease and buffer system that allows for the extraction of PCR-ready DNA from mouse tissue in as little as 15 minutes, and KAPA2G Fast Genotyping Mix with dye, containing a DNA polymerase engineered via directed evolution for high processivity and extreme speed. The combination of KAPA Express Extract and KAPA2G Fast Genotyping Mix allows for the reliable extraction and amplification of DNA fragments from mouse tissue in as little as 1 hour, as compared to ≥1 day with conventional protocols. KAPA2G Fast Genotyping Mix (2X) is a ready-to-use master mix containing all components for fast PCR with stabilizers, two inert dyes except primers and template.

Biochem/physiol Actions

KAPA2G Fast Genotyping Mix generated DNA fragments are 3′-dA-tailed and may be cloned into TA cloning vectors, or used for routine downstream analyses or applications, including restriction enzyme digestion, cloning and sequencing. Like wild-type Taq, KAPA2G Fast has 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated. Unlike existing protocols that rely on Proteinase K digestion, extractions using KAPA Express Extract are conveniently performed in a single tube, without the need for hazardous chemicals, or multiple wash steps.

KAPA2G Fast HotStart Genotyping Mix Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte


KAPA2G Fast HotStart Genotyping Mix Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Global( 1)Lieferanten
Firmenname Telefon E-Mail Land Produktkatalog Edge Rate
Sigma-Aldrich 021-61415566 800-8193336
orderCN@merckgroup.com China 51471 80

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