장기(또는, 영향을 받은 알려진 모든 장기를 명시)에 손상을 일으킬 수 있음(노출되어도 특정 표적장기 독성을 일으키지 않는다는 결정적인 노출경로가 있다면 노출경로를 기재)
특정 표적장기 독성 - 2회 노출
구분 2
경고
P260, P264, P270, P309+P311, P405,P501
예방조치문구:
P260
분진·흄·가스·미스트·증기·...·스프레이를 흡입하지 마시오.
P264
취급 후에는 손을 철저히 씻으시오.
P264
취급 후에는 손을 철저히 씻으시오.
P270
이 제품을 사용할 때에는 먹거나, 마시거나 흡연하지 마시오.
P405
밀봉하여 저장하시오.
P501
...에 내용물 / 용기를 폐기 하시오.
KAPA2G Fast HotStart Genotyping Mix C화학적 특성, 용도, 생산
용도
KAPA2G Fast HotStart? Genotyping Mix has been used in:
Genomic DNA extraction and in the preparation of master mix for PCR
Extraction and amplification from mouse tail, ear, and toe tissue
Extraction and amplification of DNA from other animal tissues
일반 설명
KAPA2G Fast HotStart? Mouse Genotyping Kits include KAPA Express Extract, a novel thermostable protease and buffer system that allows for the extraction of PCR-ready DNA from mouse tissue in as little as 15 minutes, and KAPA2G Fast Genotyping Mix with dye, containing a DNA polymerase engineered via directed evolution for high processivity and extreme speed. The combination of KAPA Express Extract and KAPA2G Fast Genotyping Mix allows for the reliable extraction and amplification of DNA fragments from mouse tissue in as little as 1 hour, as compared to ≥1 day with conventional protocols. KAPA2G Fast Genotyping Mix (2X) is a ready-to-use master mix containing all components for fast PCR with stabilizers, two inert dyes except primers and template.
Biochem/physiol Actions
KAPA2G Fast Genotyping Mix generated DNA fragments are 3′-dA-tailed and may be cloned into TA cloning vectors, or used for routine downstream analyses or applications, including restriction enzyme digestion, cloning and sequencing. Like wild-type Taq, KAPA2G Fast has 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated. Unlike existing protocols that rely on Proteinase K digestion, extractions using KAPA Express Extract are conveniently performed in a single tube, without the need for hazardous chemicals, or multiple wash steps.
