Membrane Scaffold Protein 1D1 Delta-Helix 5 Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our
Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.
Allgemeine Beschreibung
Nanodisc technology is an approach to render membrane proteins soluble in aqueous solutions in a native-like bilayer environment, where the membrane proteins remain stable and active. The Nanodisc concept is derived from high density lipoprotein (HDL) particles and their primary protein component, apolipoprotein. The Nanodisc is a non-covalent structure of phospholipid bilayer and membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1).
The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The Membrane Scaffold Protein 1D1 (MSP1D1) variant of MSP1 deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence. The MSP1D1ΔH5 variant of MSP1D1 omits the 22 amino acids of the Delta-Helix 5 sequence (H5).
Membrane Scaffold Protein 1D1 Delta-Helix 5 Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
