PI-PLC, 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
Phospholipase C, Phosphatidylinositol-specific from
Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in
T. cruzi invasion to human epithelial type 2 (HEp-2) cells.
Allgemeine Beschreibung
Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.
Biochem/physiol Actions
Used for the release of GPI anchored proteins from the membrane.
PI-PLC, 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
