DOTAP

DOTAP Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

The DOTAP (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate) Liposomal Transfection Reagent is a liposome formulation of the cationic lipid DOTAP. DOTAP can be used for the highly efficient transfection of DNA including yeast artificial chromosomes (YACs) into eukaryotic cells for transient or stable gene expression, and is also suitable for the efficient transfer of other negatively charged molecules, such as RNA, oligonucleotides, nucleotides, ribonucleo-protein (RNP) complexes, and proteins into research samples of mammalian cells.

Allgemeine Beschreibung

DOTAP Liposomal Transfection Reagent is a liposome formulation of the cationic lipid DOTAP. Unlike other liposomal reagents, it produces highly efficient transfections both in the presence and absence of serum. It can also be successfully used for in vivo applications. Mixing DOTAP with DNA results in spontaneously formed stable complexes. These complexes can be added directly to the cell culture medium. This method of DNA transfer is very gentle, avoiding the cytotoxic effects commonly encountered with lipofection or other transfection methods.

Biochem/physiol Actions

DOTAP (1,2-dioleoyl-3-trimethylammonium-propane) is a cationic lipid, used for in vitro and in vivo nucleic acid and protein delivery. The lipid composition and cell type affects the transfection potential of DOTAP. DOTAP mediates gene knockdown technique in hematopoietic stem cells (HSCs).

DOTAP Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte

DOTAP Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Firmenname	Telefon	E-Mail	Land	Produktkatalog	Edge Rate
Shochem(Shanghai) Co.,Ltd	86-21-50800795	info@shochem.com	CHINA	288	58
Zhengzhou Alfa Chemical Co.,Ltd	+8618530059196	sale04@alfachem.cn	China	13349	58
Career Henan Chemica Co	+86-0371-86658258 +8613203830695	laboratory@coreychem.com	China	30250	58
XIAMEN SINOPEG BIOTECH CO., LTD.	+86-0592-7761068 +8617350879715	changaiwei@sinopeg.com	China	183	58
Baoji Guokang Bio-Technology Co., Ltd.	0917-3909592 13892490616	gksales1@gk-bio.com	China	9316	58
BOC Sciences	16314854226; +16314854226	inquiry@bocsci.com	United States	19743	58
LEAPCHEM CO., LTD.	+86-852-30606658	market18@leapchem.com	China	43348	58
Jilin Chinese Academy of Sciences-yanshen Technology	+undefined18143011203	info@chemextension.com	China	42057	58
Aladdin Scientific	+1-+1(833)-552-7181	sales@aladdinsci.com	United States	57511	58
Hebei Weibang Biotechnology Co., Ltd	+8615350571055	Sibel@weibangbio.com	China	5848	58

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• TFX(TM) REAGENTS TRANSFECTION TRIO
• N-(2,3-DIOLEOYLOXY-1-PROPYL)TRIMETHYLAMMONIUM METHYL SULFATE
• N-[1-(2,3-DIOLEOYLOXY)PROPYL]-N,N,N-TRIMETHYLAMMONIUM METHYL-SULFATE
• TRANSFAST(TM) TRANSFECTION REAGENT
• TRANSFECTION REAGENT
• DOTAP METHOSULFATE
• DOTAP
• N-(2,3-dioleoyloxy-1-propyl)trime.-amm. me. sulfate
• N-(2,3-DIOLEOYLOXY-1-PROPYL)TRIME.-AMM. ME. SULFATE,5X0.4ML
• DOTAP methyl sulfate
• DOTAP Liposomal Transfection Reagent
• 18:1 TAP (DOTAP, MS Salt)
• DOTAP Liposomal Transfection Reagent N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate
• DOTAP Transfection Reagent
• N,N,N-Trimethyl-2,3-bis(oleoyloxy)propan-1-aminium methyl sulfate
• DOTAP MS
• 144189-73-1
• BioChemical
• Transfection & Transformation
• Molecular Biology
• Cloning and Expression
• DMG