pIEx-2 DNA - Novagen Chemische Eigenschaften,Einsatz,Produktion Methoden
Allgemeine Beschreibung
The pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected
Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pIEx-2 contains N-terminal GST?Tag, His?Tag, and S?Tag coding sequences, and a C-terminal HSV?Tag coding sequence. N-terminal vector encoded sequences can be completely removed by cloning into the PshA I site and cleaving the fusion protein with enterokinase.
pIEx-2 is also available as an
Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.
For sequencing or PCR of recombinants in pIEx-2, refer to the table below to choose the appropriate primers.
pIEx-2 DNA - Novagen Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
