pIEx-2 DNA - Novagen

pIEx-2 DNA - Novagen 구조식 이미지
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pIEx-2 DNA - Novagen
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pIEx-2 DNA - Novagen
CBNumber:
CB54817766
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0
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pIEx-2 DNA - Novagen 속성

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pIEx-2 DNA - Novagen C화학적 특성, 용도, 생산

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The pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pIEx-2 contains N-terminal GST?Tag, His?Tag, and S?Tag coding sequences, and a C-terminal HSV?Tag coding sequence. N-terminal vector encoded sequences can be completely removed by cloning into the PshA I site and cleaving the fusion protein with enterokinase.
pIEx-2 is also available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.

For sequencing or PCR of recombinants in pIEx-2, refer to the table below to choose the appropriate primers.

pIEx-2 DNA - Novagen 준비 용품 및 원자재

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pIEx-2 DNA - Novagen 공급 업체

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