TTH DNA POLYMERASE

TTH DNA POLYMERASE Struktur
CAS-Nr.
Englisch Name:
TTH DNA POLYMERASE
Synonyma:
Q-THERMORT(TM);TTH DNA POLYMERASE;RTTH DNA POLYMERASE;THERMOSTABLE REVERSE TRANSCRIPTASE
CBNumber:
CB7467709
Summenformel:
Molgewicht:
0
MOL-Datei:
Mol file

TTH DNA POLYMERASE Eigenschaften

storage temp. 
-20°C

Sicherheit

TTH DNA POLYMERASE Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

Thermus thermophilus (Tth) DNA Polymerase might be used:
  • to amplify DNA fragments by polymerase chain reaction (PCR) due to its resistance towards prolonged incubations at high temperatures (95 °C)
  • to label DNA fragments with either radiolabeled nucleotides, digoxigenin, or biotin, since this enzyme accepts modified deoxyribonucleotides as substrates
  • to efficiently transcribe RNA targets into cDNA due to its intrinsic Mn-dependent reverse transcriptase (RT) activity
  • for real time PCR

Allgemeine Beschreibung

Tth DNA Polymerase is isolated from the thermophilic eubacterium Thermus thermophilus HB8, and is purified to be free of nonspecific DNases and RNases. The enzyme is a highly processive 5′-3′ DNA polymerase, and lacks 3′-5′ exonuclease activity. The enzyme exhibits its highest activity at a pH of approximately 9 (adjusted at +25°C), and temperatures around +75°C. It is resistant to prolonged incubations at high temperatures (+95°C).

Biochem/physiol Actions

Thermus thermophilus (Tth) DNA Polymerase is a thermostable DNA polymerase with intrinsic reverse transcriptase (RT) activity. The enzyme is a highly processive 5′-3′ DNA polymerase and lacks 3′-5′ exonuclease activity (proof reading). It was found to possess a very efficient intrinsic reverse transcriptase (RT) activity in the presence of manganese ions – much higher than that demonstrated for Escherichia coli DNA polymerase I and Taq DNA Polymerase. The RT activity is not associated with RNase H activity. The elevated temperatures of Tth DNA Polymerase (optimum +55°C to +70°C, maximum +95°C) activity overcomes the problems posed by RNA secondary structure. Resulting cDNA can be amplified by PCR using the same enzyme in the presence of Mg2+-ions. The ability of Tth DNA Polymerase to perform both reverse transcription and DNA amplification at elevated temperatures allows this enzyme to be used for quantitative RT-PCR, cloning, and gene expression analysis of cellular and viral RNA. Tth DNA Polymerase is used for RT-PCR amplification of RNA up to 1kb.

TTH DNA POLYMERASE Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte


TTH DNA POLYMERASE Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Global( 32)Lieferanten
Firmenname Telefon E-Mail Land Produktkatalog Edge Rate
Yinjia (Shanghai) Bio-pharmaceutical Technology Co. , Ltd. 021-52992719 13512169707
product@yinjibio.com China 29 58
Sigma-Aldrich 021-61415566 800-8193336
orderCN@merckgroup.com China 51471 80
Beijing Solarbio Science & Tecnology Co., Ltd. 010-50973130 4009686088
3193328036@qq.com China 29797 68
Wuhan Peptide Core Biotechnology Co., Ltd 027-65317797 13667154760
1096814016@qq.com China 9907 58

  • TTH DNA POLYMERASE
  • THERMOSTABLE REVERSE TRANSCRIPTASE
  • RTTH DNA POLYMERASE
  • Q-THERMORT(TM)
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