TITAN ONE TUBE RT-PCR KIT

Uses

Titan One Tube RT-PCR Kit has been used:

• to complete cDNA synthesis?in reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
• to clone sirtuin 6 (SIRT6) into the backbone of pDsRed2-N1
• to amplify full-length and truncated cDNA fragments of?C-X-C motif chemokine 5 (CXCL5)
• to analyze RNA (total RNA, poly (A)⁺ RNA, viral RNA) from a variety of sources (animal, plant, human, viral, and bacterial). In a single tube, the Titan One Tube RT-PCR Kit will reverse transcribe RNA into cDNA,? then amplify the cDNA with a thermal cycling reaction. The enzymes AMV Reverse Transcriptase and the Expand High Fidelity PCR System are used, respectively. Depending on the primers chosen, a specific message or the entire population of transcripts can be amplified. The RT-PCR method also permits the cloning of rare messages without the need to construct cDNA libraries. The kit may be used for:Qualitative, semi quantitative, or quantitative analysis of RNA transcription levels even in single cells in combination with gel-based detection methods or ELISA-based detection methods
• for cloning of RNA up to 6 kb
• for mutation analysis at RNA level in combination with sequencing or other mutation scanning techniques

General Description

Titan^? One Tube RT-PCR Kit uses Avian Myeloblastosis Virus (AMV) reverse transcriptase for first strand cDNA synthesis and the Expand^? High Fidelity enzyme blend consisting of Taq DNA polymerase and Tgo DNA polymerase for amplification of cDNA by PCR. Tgo DNA polymerase possesses proofreading activity leading to an increased PCR fidelity. This kit reduces the error rate, allows the amplification of fragments up to 6 kb by RT-PCR, delivers increased sensitivity. It is considered faster than conventional two-step RT-PCR.