TITAN ONE TUBE RT-PCR KIT

TITAN ONE TUBE RT-PCR KIT 구조식 이미지
카스 번호:
상품명:
TITAN ONE TUBE RT-PCR KIT
동의어(영문):
TITAN ONE TUBE RT-PCR KIT
CBNumber:
CB22542191
분자식:
포뮬러 무게:
0
MOL 파일:
Mol file

TITAN ONE TUBE RT-PCR KIT 속성

저장 조건
-20°C

안전

TITAN ONE TUBE RT-PCR KIT C화학적 특성, 용도, 생산

용도

Titan One Tube RT-PCR Kit has been used:
  • to complete cDNA synthesis?in reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
  • to clone sirtuin 6 (SIRT6) into the backbone of pDsRed2-N1
  • to amplify full-length and truncated cDNA fragments of?C-X-C motif chemokine 5 (CXCL5)
  • to analyze RNA (total RNA, poly (A)+ RNA, viral RNA) from a variety of sources (animal, plant, human, viral, and bacterial). In a single tube, the Titan One Tube RT-PCR Kit will reverse transcribe RNA into cDNA,? then amplify the cDNA with a thermal cycling reaction. The enzymes AMV Reverse Transcriptase and the Expand High Fidelity PCR System are used, respectively. Depending on the primers chosen, a specific message or the entire population of transcripts can be amplified. The RT-PCR method also permits the cloning of rare messages without the need to construct cDNA libraries. The kit may be used for:Qualitative, semi quantitative, or quantitative analysis of RNA transcription levels even in single cells in combination with gel-based detection methods or ELISA-based detection methods
  • for cloning of RNA up to 6 kb
  • for mutation analysis at RNA level in combination with sequencing or other mutation scanning techniques

일반 설명

Titan? One Tube RT-PCR Kit uses Avian Myeloblastosis Virus (AMV) reverse transcriptase for first strand cDNA synthesis and the Expand? High Fidelity enzyme blend consisting of Taq DNA polymerase and Tgo DNA polymerase for amplification of cDNA by PCR. Tgo DNA polymerase possesses proofreading activity leading to an increased PCR fidelity. This kit reduces the error rate, allows the amplification of fragments up to 6 kb by RT-PCR, delivers increased sensitivity. It is considered faster than conventional two-step RT-PCR.

TITAN ONE TUBE RT-PCR KIT 준비 용품 및 원자재

원자재

준비 용품


TITAN ONE TUBE RT-PCR KIT 공급 업체

글로벌( 15)공급 업체
공급자 전화 이메일 국가 제품 수 이점
Sigma-Aldrich 021-61415566 800-8193336
orderCN@merckgroup.com China 51471 80

Copyright 2019 © ChemicalBook. All rights reserved