Monoclonal Anti-Biotin;Monoclonal Anti-Biotin antibody produced in mouse;Monoclonal Anti-Biotin–FITC antibody produced in mouse;Monoclonal Anti-Biotin–Cy3? antibody produced in mouse;Monoclonal Anti-Biotin–Agarose antibody produced in mouse;Monoclonal Anti-Biotin?Peroxidase antibody produced in mouse;Monoclonal Anti-Biotin–Alkaline Phosphatase antibody produced in mouse
Mouse monoclonal anti-biotin antibody was used to identify by western blot S-nitrosylated proteins that had been labeled with a biotin moeity based on the biotin-switch method. No reactivity was visible on the blots when the biotin switch method was inhibited by treatment with 100 mM dithiothreitol showing that this antibody shows no unspecific cross reactivity. The mouse monoclonal anti-biotin antibody does cross react with Arabidopsis plant by western blot, no cross reactivity with unbiotinylated proteins. In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
일반 설명
Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. The use of monoclonal anti-biotin IgG can enhance the sensitivity of such avidin-biotin immunoassays. The monoclonal antibody functions by selectively enlarging and bridging the avidin-biotin-enzyme complexes, thereby increasing the signals from substrate conversion. Furthermore, this antibody can be used in many other applications where biotin may be introduced as target label.
Biochem/physiol Actions
Biotin may participate in the replication and transcription of DNA. It acts as a coenzyme in the fatty acid biosynthesis, glyconeogenesis, and catabolism of several branched-chain amino acids and odd-chain fatty acids.
