β-Gal ELISA

β-Gal ELISA C화학적 특성, 용도, 생산

용도

For research use only. Not for use in diagnostic procedures.
The β-Gal ELISA is used to quantitatively measure β-Gal expression in eukaryotic cells transfected with a plasmid bearing a β-Gal-encoding reporter gene. The β-Gal ELISA may also be used for quantification of fusion proteins, produced by in-frame cloning into an appropriate β-Gal encoding DNA construct.

일반 설명

β-Gal (β-D-galactoside galactohydrolase, EC 3.2.1.23) from E. coli is specifically detected by the β-Gal ELISA. The β-Gal enzyme from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.

Contents:

• β-Gal Enzyme (from E. coli)
• Anti-β-Gal-digoxigenin
• Anti-DIG-peroxidase
• POD Substrate
• Substrate Enhancer
• Washing Buffer concentrate, 10x
• Sample Buffer
• Lysis Buffer concentrate, 5x
• Two Microplates, strip frame with 12 modules of 8 wells, precoated with anti-β-Gal
• Self-adhesive Plate Cover Foils (3)

Biochem/physiol Actions

Promoter activity in transfected mammalian cells is generally studied by linking the promoter sequence to a gene that encodes an easily detectable “reporter” protein, such as chloramphenicol acetyltransferase (CAT), β-galactosidase (β-Gal), or luciferase (Luc).The E. coli lacZ gene, encoding the enzyme β-galactosidase (β-Gal), has become one of the standard reporter genes used in transfection experiments. Traditionally, β-Gal activity is measured using colorimetric assays with o-nitrophenol-β-D-galactopyranoside (ONPG) or chlorophenol red β-D-galactopyranoside (CPRG) as substrates. These assays are not sensitive enough to detect small amounts of the enzyme. Another disadvantage is that in order to enable discrimination between the endogenous lysosomal β-Gal activity and the bacterial enzyme, the colorimetric assays must be performed at an alkaline pH. In contrast, β-Gal ELISA specifically detects the bacterial enzyme, but not the analogous lysosomal β-galactosidase.

β-Gal ELISA 준비 용품 및 원자재

원자재

준비 용품

β-Gal ELISA 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80