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ChemicalBook >> Duolink In Situ Detection Reagents Brightfield

Duolink In Situ Detection Reagents Brightfield

Duolink In Situ Detection Reagents Brightfield price.
2 prices
Selected condition:
Brand
  • Sigma-Aldrich
Package
  • 30REACTIONS
  • 100REACTIONS
  • ManufacturerSigma-Aldrich
  • Product numberDUO92012
  • Product descriptionDuolink® In Situ Detection Reagents Brightfield
  • Packaging100REACTIONS
  • Price$970
  • Updated2023-06-20
  • Buy
  • ManufacturerSigma-Aldrich
  • Product numberDUO92012
  • Product descriptionDuolink® In Situ Detection Reagents Brightfield
  • Packaging30REACTIONS
  • Price$655
  • Updated2023-06-20
  • Buy
Manufacturer Product number Product description Packaging Price Updated Buy
Sigma-Aldrich DUO92012 Duolink® In Situ Detection Reagents Brightfield 100REACTIONS $970 2023-06-20 Buy
Sigma-Aldrich DUO92012 Duolink® In Situ Detection Reagents Brightfield 30REACTIONS $655 2023-06-20 Buy

Properties

storage temp. :-20°C

Safety Information

Symbol(GHS): GHS hazard pictogramsGHS hazard pictogramsGHS hazard pictogramsGHS hazard pictograms
Signal word: Danger
Hazard statements:
Code Hazard statements Hazard class Category Signal word Pictogram P-Codes
H225 Highly Flammable liquid and vapour Flammable liquids Category 2 Danger GHS hazard pictograms P210,P233, P240, P241, P242, P243,P280, P303+ P361+P353, P370+P378,P403+P235, P501
H315 Causes skin irritation Skin corrosion/irritation Category 2 Warning GHS hazard pictograms P264, P280, P302+P352, P321,P332+P313, P362
H317 May cause an allergic skin reaction Sensitisation, Skin Category 1 Warning GHS hazard pictograms P261, P272, P280, P302+P352,P333+P313, P321, P363, P501
H318 Causes serious eye damage Serious eye damage/eye irritation Category 1 Danger GHS hazard pictograms P280, P305+P351+P338, P310
H331 Toxic if inhaled Acute toxicity,inhalation Category 3 Danger GHS hazard pictograms P261, P271, P304+P340, P311, P321,P403+P233, P405, P501
H341 Suspected of causing genetic defects Germ cell mutagenicity Category 2 Warning P201,P202, P281, P308+P313, P405,P501
H412 Harmful to aquatic life with long lasting effects Hazardous to the aquatic environment, long-term hazard Category 3 P273, P501
Precautionary statements:
P210 Keep away from heat/sparks/open flames/hot surfaces. — No smoking.
P273 Avoid release to the environment.
P280 Wear protective gloves/protective clothing/eye protection/face protection.
P303+P361+P353 IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

Description

Duolink?proximity ligation assay(PLA?) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink? In Situ Brightfield Protocol to use this product.

Visit our Duolink? PLA Resource Center for information on how to run a Duolink? experiment, applications, troubleshooting, and more.

To perform a complete Duolink? PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink? PLA probes. Analysis is carried out using HRP for brightfield detection.

Specificity
Brightfield detection reagents are used for samples with significant autofluorescence.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink? in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink? projects

View full Duolink? product list

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