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ChemicalBook Optimization Suppliers |
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| 融点 | 243 °C (dec.) | | 比旋光度 | D25 (per mg N) -0.47° (c = 5) | | 貯蔵温度 | -20°C | | 溶解性 | Reconstitute in 10mM acetate, pH 5.0 with or without 15mM calcium chloride | | 外見 | solid | | 色 | white | | PH | pH : 8.0 | | 水溶解度 | Soluble in water. | | Sensitive | Moisture Sensitive | | EPAの化学物質情報 | Nuclease, ribo- (9001-99-4) |
| Sフレーズ | 22-24/25 | | WGK Germany | 3 | | RTECS 番号 | RF0760000 | | F | 10-21 | | TSCA | Yes | | HSコード | 35079090 |
| | リボヌクレアーゼA Usage And Synthesis |
| 外観 | 液体 | | 溶解性 | 水及びエタノールに任意の割合で混和する。 | | 解説 | リボ核酸（RNA）の分子内リン酸ジエステル結合を切断する酵素で、RNアーゼ（RNase）と略記する。動植物から細菌、カビに至るまで広く分布する。代表的なものに、ウシの膵臓(すいぞう)RNアーゼA、江上不二夫(えがみふじお)らがタカジアスターゼ末から精製したRNアーゼT1がある。いずれも分子量1万強の比較的小さなタンパク質で、熱にきわめて安定である。そのアミノ酸配列、物理化学的性質、作用機序などが詳細に研究された。厳密な基質特異性を有するものが多く、RNアーゼAはピリミジンヌクレオチドに特異的であり、またRNアーゼT1はグアニル酸残基に特異的で、いずれもその3´-リン酸体を切断末端に生ずる。反応は二段階よりなり、第一段階ではRNA鎖中の標的残基の3´-リン酸と隣の残基の5´-ヒドロキシ基との間の結合を切断し、2´位と3´位の間で環状リン酸エステルを中間体として生じる。続いて加水分解により3´-リン酸末端を与えるが、生成物はモノおよびオリゴヌクレオチドで、無機リン酸は生じない。その厳密な特異性は、1965年ホリーらによるRNA配列決定の際の有力な武器となった。また、第一段階の反応は可逆的であるため、既知配列のオリゴヌクレオチドの合成にも利用される。 | | 使用上の注意 | 使用時の濃度は、10～20μg/mlが適当である。あまり高い濃度にするとプラスミドDNAなどは''Nick''が入ることがある。 | | 化学的特性 | Lyophilized | | 使用 | Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA.Ribonuclease A from bovine pancreas has been used in a study to assess hybridase activity of human ribonuclease-1. Ribonuclease A from bovine pancreas has also been used in a study to investigate particle-based and monolithic columns for cation exchange protein displacement chromatography. | | 使用 | Ribonuclease A (RNase A) is a pancreatic ribonuclease that cleaves single-stranded RNA. RNase A, and several homologs, may be useful as cancer therapeutics due to their cytotoxic effects on cancer cells. Rnase A is useful in the elucidation of protein structure and absorbance. RNase A has been used as a model system in the study of computer-aided drug design . Product R2638 has scrambled disulfide bonds and is from bovine pancreas. | | 使用 | Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the mechanism of heavy metal ions on RNase activity. Ribonuclease A from bovine pancreas has also been used in a study to investigate the performance of oligomeric poly(diallyldimethylammonium chloride) as displacer for cation-exchange displacement chromatography of proteins. | | 一般的な説明 | Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils. | | Biochem/physiol Actions | Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. | | 純化方法 | Purify it by (NH4)2SO4 fractionation, followed by PC cellulose chromatography and affinity chromatography (using Sepharose 4B to which (G)n is covalently bonded). [Schmukler et al. J Biol Chem 250 2206 1975.] Ribozymes. These are ribonucleic acids which act like protein enzyme in catalyzing the making and breaking of peptide bonds as well as catalyzing reactions and cleavage of DNA and RNA molecules. The short RNAs are being intensively studied (see RNAi below). MicroRNAs (miRNA, see also below) are ubiquitous and are also genetically produced. They are involved in numerous reactions from splicing RNA, e.g. mRNA (messenger RNA) to controlling transcription of DNA to RNA, and translation of RNA to protein. Each miRNA is capable of being involved in a small number to hundreds of interactions with nucleic acids and with proteins. |
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