|
ChemicalBook Optimization Suppliers |
| 名前: |
Sigma-Aldrich |
| 電話番号: |
021-61415566 800-8193336 |
| 電子メール: |
orderCN@merckgroup.com |
| 名前: |
Energy Chemical |
| 電話番号: |
021-58432009 400-005-6266 |
| 電子メール: |
marketing@energy-chemical.com |
| 名前: |
amyjetsci |
| 電話番号: |
027-59626688 18771149750 |
| 電子メール: |
sales@amyjet.com |
|
| | Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* 製品概要 |
| 化学名: | | | 英語化学名: | Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* | | 别名: | Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* | | CAS番号: | 136632-31-0 | | 分子式: | | | 分子量: | 0 | | EINECS: | | | カテゴリ情報: | | | Mol File: | Mol File | ![Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)*]() |
| | Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* 物理性質 |
| | Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* Usage And Synthesis |
| 使用 | For nonradioactive DNA labeling such as random priming, PCR labeling, or nick translation.
- Biotin-16-dUTP is used as asubstrate for: Terminal Transferase
- DNA polymerase I (holoenzyme and Klenow fragment)
- Taq DNA polymerase
- Reverse transcriptase (e.g., Transcriptor)
Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% Biotin-16-dUTP and 65% dTTP, as well as in PCR.
The nucleotide also serves as a substrate for Terminal Transferase in 3′-end labeling.
- Biotin-labeled DNA can be detected with: Streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate (CSPD, CDP-Star) or a color substrate
- Biotin Luminescence Detection Kit
| | 一般的な説明 | Biotin is a water-soluble vitamin which functions as a coenzyme for carboxylases. | | Biochem/physiol Actions | Biotin influences gene regulation and chromatin structure by associating with lysine residues in histones. It influences the actions of adaptive immune T and natural killer cells. Deficiency of biotin in mice has indicated enhanced inflammation. Studies have also indicated heightened inflammatory response of human dendritic cells in conditions of biotin deficiency. |
|