荧光钙探针 Fura 2-AM

荧光钙探针 Fura 2-AM

中文名称荧光钙探针 Fura 2-AM
中文同义词钙离子荧光探针;钙荧光探针FURA-2, AM;钙离子荧光探针 Fura 2-AM;荧光钙探针 Fura 2-AM;AM荧光探针;FURA-2/AM荧光探针;FURA 2-AM 1MM/L DMSO SOLUTION
英文名称Fura 2-AM
英文同义词FURA 2/AM;FURA-2 PENTAKIS(ACETOXYMETHYL) ESTER;FURA-2/AM, FOR FLUORESCENCE;FURA 2-AM SPECIAL PACKAGING;FURA 2/AM in Solution;fura 2-am yellow powder;Fura?-AM;FURA2/AM *1SET = 10x100UG**
CAS号108964-32-5
分子式C44H47N3O24
分子量1001.85
EINECS号
相关类别荧光探针,标记,颗粒和染色;分析试剂-显色剂;生化试剂;荧光染料;Benzofuran
Mol文件108964-32-5.mol
结构式荧光钙探针 Fura 2-AM 结构式

荧光钙探针 Fura 2-AM 性质

熔点>250℃
沸点975.9±75.0 °C(Predicted)
密度1.400±0.06 g/cm3(Predicted)
储存条件−20°C
溶解度可溶于DMSO
酸度系数(pKa)1.89±0.50(Predicted)
形态粉末
颜色粘黄色
最大波长(λmax)370nm
敏感性Light Sensitive
BRN8183748
生物领域应用Calcium indicator; monitoring membrane potential; treating ischemia
CAS 数据库108964-32-5(CAS DataBase Reference)

荧光钙探针 Fura 2-AM 用途与合成方法

Fura-2 AM 是一种高亲和力,细胞内,紫外光激发和 UV 比率荧光 Ca2+ 指示剂。

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Fura-2 AM diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid.
1. First, prepare the 1 mM Fura-2 AM stock by adding 50 µL of DMSO to a 50 µg vial. It is important to use dry DMSO packed under nitrogen and it is necessary to remove the DMSO with a needle by puncturing the septum to prevent hydration of the DMSO. After preparing the Fura-2 AM solution keep it in a dark dry place. Fura-2 AM in DMSO is stable at RT for 24 hours and is stable at -20 degrees in a dry container for several months.
2. Aliquot 2 mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 µL of Fura-2 AM stock to generate a 1µM Fura-2 AM solution. Vortex the solution vigorously for 1 min.
3. Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish.
4. Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Time the incubation precisely.
5. Prepare a 35 mm dish containing 2 mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish.
6. Mount the coverslip on the imaging chamber.

安全信息

WGK Germany3
F8-10

MSDS信息

提供商 语言
英文
更新日期产品编号产品名称CAS号包装价格
2024/01/25HY-101897荧光钙探针 Fura 2-AM
Fura-2 AM
108964-32-51mg5000元

荧光钙探针 Fura 2-AM 上下游产品信息

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