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| 化学名: | ビシンコニン酸二ナトリウム水和物 | | 英語化学名: | Bicinchoninic Acid Disodium Salt | | 别名: | SodiumBicinchoninate;BCA DISODIUM SALT;BICINCHONINIC ACID DISODIUM SALT;DI-SODIUM 2,2'-BICINCHONINATE;DISODIUM 2,2'-BIQUINOLINE-4,4'-DICARBOXYLATE;2,2'-BICINCHONINIC ACID DISODIUM SALT;2,2'-BIQUINOLINE-4,4'-DICARBOXYLIC ACID DISODIUM SALT;2,2'-BIQUINOLINE-4,4'-DICARBOXYLIC ACID DISODIUM SALT TRIHYDRATE | | CAS番号: | 979-88-4 | | 分子式: | C20H13N2NaO4 | | 分子量: | 368.32 | | EINECS: | 629-761-2 | | カテゴリ情報: | marker | | Mol File: | 979-88-4.mol |  |
| 融点 | 300℃ | | 貯蔵温度 | Inert atmosphere,2-8°C | | 溶解性 | 0.5% sodium bicarbonate: soluble50mg/mL | | 外見 | Powder/Chunks | | 色 | white to light yellow | | 水溶解度 | Soluble in water. | | 安定性： | Hygrscopic | | InChI | InChI=1S/C20H12N2O4.Na.H/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18;;/h1-10H,(H,23,24)(H,25,26);; | | InChIKey | AUPXFICLXPLHBB-UHFFFAOYSA-L | | SMILES | C(C1=CC(C2=NC3=CC=CC=C3C(C(=O)O)=C2)=NC2=CC=CC=C12)(=O)O.[NaH] | | CAS データベース | 979-88-4(CAS DataBase Reference) | | EPAの化学物質情報 | [2,2'-Biquinoline]-4,4'-dicarboxylic acid, disodium salt (979-88-4) |
| | ビシンコニン酸二ナトリウム水和物 Usage And Synthesis |
| 外観 | 白色～うすい黄色粉末～結晶 | | 説明 | 2,2’-Biquinoline-4,4’-dicarboxylic acid (BCA) is a colorimetric reagent for the detection of copper (Cu+). BCA and Cu+ form a stable 2:1 complex that has an absorbance maximum at 562 nm. It is commonly used as a reagent in the colorimetric BCA protein quantitation assay in which Cu2+ is converted to Cu+ as a function of protein concentration under alkaline conditions. | | 化学的特性 | White to light yellow powder | | 使用 | - Bicinchoninic acid disodium salt hydrate has been used to monitor the levels of reducing sugars, thereby helping in the detection of chitinases.
- It has also been used to determine the amount of cellulose reducing ends (micromoles/g cellulose).
- It has been used for determining protein concentration.
| | 使用 | For determination of Cu and protein assays.Bicinchoninic acid disodium salt is useful for the determination of copper. It is also used for the determination of protein concentration. | | 主な応用 | 2,2'-Bicinchoninic Acid Disodium Salt is a photometric reagent for protein assay. It is used for determination of reducing sugar and protein via Cu(I). Also a specific reagent for Cu(I) with sensitivity similar to that of neocuproin. | | Biochem/physiol Actions | Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+), in presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration. |
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