车叶草苷酸是环烯醚萜类物质,是诺尼果汁中重要的活性成分,具有抗肿瘤、抗病毒、抗氧化、抗畸变等活性,与人体健康密切相关。
取10kg白花蛇舌草加入10倍量体积浓度为75%乙醇回流提取2次,每次1h,60℃减压浓缩至相对密度为1.10-1.20;往浓缩液中加入重量百分比为0.5%的活性炭脱色,高速离心,取上清,真空干燥,得白花蛇舌草粗提物;将粗提物采用30%甲醇溶解,以ODS开口柱为固定相,30%甲醇水溶液洗脱,回收甲醇后,真空干燥。干燥后样品采用5%甲醇溶解,采用高速逆流色谱法分离获得车叶草苷酸。制备条件:YMC-PackODS-A色谱柱(20×250mm,5μm)为色谱柱,以体积浓度为5%的甲醇为流动相,等梯度洗脱60min;检测波长235nm;进样体积100μL。
Asperulosidic Acid (ASPA) 车叶草苷酸是一种具有生物活性的环烯醚萜苷,从白花蛇舌草 (Hedyotis diffusa Willd) 的草药中提取的。 Asperulosidic Acid (ASPA) 具有抗肿瘤,抗氧化和抗炎作用。
Asperulosidic Acid (ASPA) 通过抑制 NF-κB 和丝裂原活化蛋白激酶 (MAPK) 信号通路抑制炎性细胞因子(TNF-α,IL-6) 释放发挥抗炎作用。
Asperulosidic Acid (ASPA) (40-160 μg/mL; pre- 1 hour) significantly down-regulates the mRNA levels of TNF-α and IL-6 in LPS-induced RAW 264.7 cells compared with the group treated with LPS alone .
Asperulosidic Acid (ASPA) (40-160 μg/mL; pre- 1 hour) decreases IκB-α phosphorylation in a concentration-dependent manner, decreases Erk1/2 phosphorylation at all concentration levels, but there was no effect on p-p38 .
RT-PCR
Cell Line:
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RAW 264.7 cells
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Concentration:
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40 μg/mL, 80 μg/mL, and 160 μg/mL
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Incubation Time:
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Pre-treatment 1 hour
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Result:
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Decreased TNF-α and IL-6 mRNA expression.
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Western Blot Analysis
Cell Line:
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RAW 264.7 cells
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Concentration:
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40 μg/mL, 80 μg/mL, and 160 μg/mL
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Incubation Time:
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Pre-treatment 1 hour
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Result:
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Decreased IκB-α phosphorylation and Erk1/2 phosphorylation.
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