NSC 95397
| 中文名称 | NSC 95397 |
|---|---|
| 中文同义词 | 2,3-双(2-羟基乙硫基)-1,4-萘二酮;化合物NSC 95397;NSC 95397,CDC25双特异性磷酸酶抑制剂;CDC25 INHIBITOR IV, NSC 95397 CALBIOCHEM |
| 英文名称 | NSC 95397 |
| 英文同义词 | 2,3-BIS(2-HYDROXYETHYLTHIO)-1,4-NAPHTHALENEDIONE;2,3-BIS[(2-HYDROXYETHYL)THIO]-1,4-NAPHTHOQUINONE;2,3-BIS[(2-HYDROXYETHYL)THIOL]-1,4-NAPHTHOQUINONE;1,4-Naphthalenedione, 2,3-bis[(2-hydroxyethyl)thio]-;Cdc25 Inhibitor IV, NSC 95397;NSC 95397;Cdc25 Inhibitor IV, NSC 95397 - CAS 93718-83-3 - Calbiochem;Apoptosis,NSC-95397,Inhibitor,NSC 95397,inhibit,NSC95397,Phosphatase |
| CAS号 | 93718-83-3 |
| 分子式 | C14H14O4S2 |
| 分子量 | 310.39 |
| EINECS号 | |
| 相关类别 | Protein Phosphatase |
| Mol文件 | 93718-83-3.mol |
| 结构式 |  |
NSC 95397 性质
| 储存条件 | -20°C |
|---|---|
| 溶解度 | 二甲基亚砜：16 毫克/毫升 |
| 形态 | 橙色固体 |
| 颜色 | 橙色，固体 |
| 稳定性 | 自购买之日起 1 年内保持稳定。 DMSO 或乙醇溶液可在 -20°C 下保存长达 1 个月。 |
NSC 95397 是一种有效的、选择性 Cdc25 双特异性磷酸酶抑制剂，对于 Cdc25A、Cdc25B 和 Cdc25C 的Ki值分别为32 nM、96 nM 和 40 nM。NSC 95397 对 hCdc25A、hCdc25C 和 hCdc25B 的IC50值分别为22.3 nM、56.9 nM和125 nM。NSC 95397 抑制 mitogen-activated protein kinase phosphatase-1 (MKP-1) 和增殖，并通过 MKP-1 和 ERK1/2 途径诱导结肠癌细胞凋亡。
| Target | Value |
|
MKP-1
() | |
|
Cdc25A
(Cell-free assay) | 22.3 nM |
|
Cdc25A
(Cell-free assay) | 32 nM(Ki) |
|
Cdc25C
(Cell-free assay) | 40 nM(Ki) |
|
Cdc25B
(Cell-free assay) | 56.9 nM |
NSC 95397 (0, 10, and 20 µM; 24 hour) decreases the cell viability of three colon cancer cell lines SW480, SW620, and DLD-1 in a concentration-dependent manner.
NSC 95397(10 μM; 24 hour) upregulates p21 while downregulates CDK4 and CDK6 were d in all three colon cancer cell lines SW480, SW620, and DLD-1 cells.
NSC 95397 (10 μM; 24 hour) reduces the phosphorylation of retinoblastoma protein (Rb) on Ser795 and Ser807/811.
NSC 95397 (20 μM; 24 hours) significantly increases cleaved caspase-9, -3, -7 and PARP levels.
NSC 95397 (10 μM; 6 hours) enhances the phosphorylation of its downstream ERK1/2 at Thr202/Tyr 204.
Cell Viability Assay
| Cell Line: | Human colon cancer cell lines, SW480, SW620, and DLD-1 |
| Concentration: | 0, 10, and 20 µM |
| Incubation Time: | 24 hours |
| Result: | The IC 50 values of NSC 95397 for the cell growth of SW480, SW620, and DLD-1 cells were 9.9, 14.1 and 18.6 μM, respectively. |
Western Blot Analysis
| Cell Line: | SW480, SW620, and DLD-1 cells |
| Concentration: | 10 μM |
| Incubation Time: | 24 hours |
| Result: |
p21 was upregulated while CDK4 and CDK6 were downregulated.
Rduced the phosphorylation of Rb on Ser795 and Ser807/811 |
安全信息
| 危险品标志 | Xi |
|---|---|
| WGK Germany | 3 |
| 危险等级 | IRRITANT |
| 提供商 | 语言 |
|---|---|
|
英文
|
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2024/04/30 | HY-108543 | NSC 95397 NSC 95397 | 93718-83-3 | 5mg | 1200元 |
| 2024/04/30 | HY-108543 | NSC 95397 NSC 95397 | 93718-83-3 | 10mM * 1mLin DMSO | 1320元 |