4-硝基苯-α-D-吡喃半乳糖苷
| 中文名称 | 4-硝基苯-α-D-吡喃半乳糖苷 |
|---|---|
| 中文同义词 | 4-硝基苯基-α-D-吡喃半乳糖苷;4-硝基苯-α-D-吡喃半乳糖苷;对硝基苯基-Α-D-吡喃半乳糖苷/4-硝基苯基-Α-D-吡喃半乳糖苷;-硝基苯基Α-D-吡喃半乳糖苷[Α-D-牛乳糖用培养基];4-硝基苯基-Α-D-吡喃半乳糖苷|4-NITROPHENYL Α-D-GALACTO-PYRAN-OSIDE;4-NITROPHENYL ALPHA-D-GALACTOPYRANOSIDE 4-硝基苯-Α-D-吡喃半乳糖苷;4-硝基苯-Α-D-半乳糖苷(PNPG);PNPG,对硝基苯-Α-D-吡喃葡萄糖苷 |
| 英文名称 | 4-NITROPHENYL-ALPHA-D-GALACTOPYRANOSIDE |
| 英文同义词 | 4-Nitrophenyl alpha-D-Galactopyranoside NSC 89286;4-Nitrophenyl α-D-galactooside;pyran4-Nitrophenyl α-D-Galactopyranoside [Substrate for α-D-Galactosidase];alpha-d-galactopyranoside,4-nitrophenyl;4-NITROPHENYL-ALPHA-D-GALACTOPYRANOSIDE |
| CAS号 | 7493-95-0 |
| 分子式 | C12H15NO8 |
| 分子量 | 301.25 |
| EINECS号 | 231-343-6 |
| 相关类别 | 其他生化试剂;糖类（碳水化合物）;生化试剂;糖类化合物;氨基酸;植物提取物;糖化学分析标准品;生化试剂-酶类;分析试剂-显色剂;通用生化试剂-酶与辅酶;Substrates;13C & 2H Sugars;Biochemistry;Galactose;Glycosides;Sugars;Enzyme substrates;Carbohydrates & Derivatives;酶底物;substrate;酶类;酶与辅酶;糖苷水解酶底物;其它 |
| Mol文件 | 7493-95-0.mol |
| 结构式 |  |
4-硝基苯-α-D-吡喃半乳糖苷 性质
| 熔点 | 166-169 °C |
|---|---|
| 沸点 | 582.2±50.0 °C(Predicted) |
| 密度 | 1.599±0.06 g/cm3(Predicted) |
| 折射率 | 239 ° (C=1, H2O) |
| 储存条件 | -20°C |
| 溶解度 | 甲醇（微溶）、水（微溶） |
| 酸度系数(pKa) | 12.55±0.70(Predicted) |
| 形态 | 固体 |
| 颜色 | 白色至类白色 |
| BRN | 92214 |
| InChI | InChI=1/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12+/s3 |
| InChIKey | IFBHRQDFSNCLOZ-IIRVCBMXSA-N |
| SMILES | O(C1C=CC(N(=O)=O)=CC=1)[C@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O |&1:10,12,15,17,19,r| |
| LogP | -0.550 (est) |
| CAS 数据库 | 7493-95-0(CAS DataBase Reference) |
4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) 是一种 4-硝基苯基 (pNP) 糖吡喃糖苷的人工底物，用于检测 α-半乳糖苷酶活性。当 4-Nitrophenyl α-D-galactopyranoside 用作底物时，释放的 pNP 量会显着增加。
Using 4-nitorophenol (pNP)-glycopyranosides as pseudosubstrates, the amount of liberated pNP from the 4-Nitrophenyl α-D-galactopyranoside is significantly higher than that from the other pNP-glycopyranosides at pH 4.0 whereas no activity was detected at pH 7.0.
The digestion assays using 4-nitrophenyl (pNP) glycopyranosides as artificial substrates shows that yieldin liberated galactose residues from 4-Nitrophenyl α-D-galactopyranoside mainly at pH 4.0, i.e. the pH level where yield threshold is decreased at most.
用途
α-D-半乳糖苷酶（α-D-Galactosidase）的底物。结合到大肠杆菌乳糖蛋白载体上。α-D-牛乳糖用培养基安全信息
| 危险品标志 | Xi |
|---|---|
| 危险类别码 | 36/37/38 |
| 安全说明 | 22-24/25-36-26 |
| WGK Germany | 3 |
| F | 3-10-21 |
| 海关编码 | 29400090 |
| 存储类别 | 11 - 可燃固体 |
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