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ChemicalBook Optimization Suppliers |
| 名前: |
Alfa Aesar |
| 電話番号: |
400-6106006 |
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saleschina@alfa-asia.com |
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| | フルオレスカミン Usage And Synthesis |
| 外観 | 白色〜黄色, 結晶性粉末〜粉末 | | 溶解性 | ジエチルエーテル及びアセトンに溶け、水にほとんど溶けない。 | | 解説 | 4-phenylspiro[furan-2(3H),1′(3′H)-isobenzofuran]-3,3′-dione．C17H10O4(278.27)．O-(α-ヒドロキシシンナモイル)安息香酸のジメチルアミノメチレン化，ついで酸性条件下，脱ジメチルアミノ基を伴う分子内環化で合成される．微黄色の結晶．融点154～155 ℃．フルオレスカミンは，アセトン，ジオキサンなど有機溶媒に可溶，水に難溶．pH 7～9の水溶液中で第一級アミンと反応して緑色の強い蛍光(475 nm)を発する．第一級アミン，アミノ酸，ペプチド，タンパク質の超微量蛍光分析に用いられ，その際，アンモニアの影響を受けない．また，蛍光ラベル化試薬として用いる．森北出版「化学辞典（第2版） | | 用途 | アミノ酸、ペプチド、第一級アミンの蛍光定量用。 | | 使用上の注意 | 不活性ガス封入 | | 化学的特性 | white powder | | 使用 | For fluorescent detection of amino acids, peptides and proteins.Fluorescamine plays an important role as a reagent for the detection of amines, peptides and proteins. It is also used as a substrate for measuring protease activity. It is utilized in the detection and quantitation of residual aminopenicillins and sulfonamides in honey by HPLC. It finds application in the determination of lisinopril in human plasma and urine. | | 使用 | Fluorescamine is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Reaction takes place under mild conditions. Low background due to hydrolysis. | | 使用 | Analytical reagent. | | Synthesis Reference(s) | Journal of the American Chemical Society, 94, p. 5927, 1972 DOI: 10.1021/ja00771a084 | | 純化方法 | Fluram is a non-fluorescent reagent that reacts with primary amines to form highly fluorescent compounds. Purify it by dissolving (~1g) in Et2O/*C6H6 (1:1, 180 mL), washing with 1% aqueous NaHCO3 (50mL), drying (Na2SO4), and evaporating in a vacuum. Dissolve the residue in warm CH2Cl2 (5mL), dilute with Et2O (12mL) and refrigerate. Collect the solid and dry it in a vacuum. IR (CHCl3): 1810, 1745, 1722, 1625 and 1600 cm-1, and 1HNMR (CDCl3): 8.71 (s, -OHC=). [Weigele et max al. J Am Chem Soc 94 5927 1972, Weigele et al. J Org Chem 41 388 1976, Lai Methods Enzymol 47 236 1977.] Forskolin (Colforsin, Coleonol, 5-[acetyloxy]-3-ethenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7, 10a-penta-methyl-[3R -{3 |
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