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| | アルカリホスファターゼ (子ウシ腸粘膜由来) 製品概要 |
| 化学名: | アルカリホスファターゼ (子ウシ腸粘膜由来) | | 英語化学名: | Alkaline Phosphatase | | 别名: | PHOSPHATASE, ALKALINE TYPE XXX FROM*CALF INTESTINAL;ALKALINE PHOSPHATASE YELLOW LI;PHOSPHATASE ALKALINE TYPE XV FROM BOVINE PLACENTA;PHOSPHATASE, ALKALINE TYPE XVIII FROM*PO RCINE INTES;PHOSPHATASE ALK. FROM CALF INTEST. MUC., STAB., ~1000 U/MG*;PHOSPHATASE ALKALINE TYPE VII-S FROM*BOV INE INTESTI;PHOSPHATASE, ALKALINE TYPE VII-NT;PHOSPHATASE, ALKALINE FROM SHRIMP | | CAS番号: | 9001-78-9 | | 分子式: | C21H36N8O6 | | 分子量: | 496.56054 | | EINECS: | 232-631-4 | | カテゴリ情報: | HPLC Fittings;SSI Fittings;Stainless Steel Fittings;enzyme;CAP | | Mol File: | 9001-78-9.mol |  |
| | アルカリホスファターゼ (子ウシ腸粘膜由来) 物理性質 |
| 比重(密度) | 1.12 g/mL at 20 °C | | 貯蔵温度 | -20°C | | 外見 | suspension | | 色 | white | | 比重 | 1 | | 水溶解度 | It is soluble in water. | | 安定性： | Stability Incompatible with strong oxidizing agents | | EPAの化学物質情報 | Alkaline phosphatase (9001-78-9) |
| 主な危険性 | B,Xi | | Rフレーズ | 36/37/38 | | Sフレーズ | 26-36-24/25-22-23 | | WGK Germany | 3 | | F | 3-10 | | TSCA | Yes | | HSコード | 35079090 | | 毒性 | An enzyme with an
alkaline pH optimum that hydrolyzes phosphate monoesters.
Elevation of its activity in the serum usually indicates obstructive
jaundice, Paget’s disease (osteitis deformans), or bone
carcinoma. |
| | アルカリホスファターゼ (子ウシ腸粘膜由来) Usage And Synthesis |
| 外観 | 液体 | | 解説 | ALPともいう。有機化合物であるリン酸エステルを加水分解する酵素。高等動物の場合には，臓器特異性をもった ALPのアイソザイム (同位酵素) が存在する。生理作用は不明であるが，血清中にはアルカリ性と酸性の2種のホスファターゼがあり，このうちアルカリ性のものは肝臓や胆道，骨などに由来し，これらの臓器に疾患があると血清中に増量する。この性質を利用して，肝機能検査などに広く用いられる。
ブリタニカ国際大百科事典 小項目事典 ブリタニカ | | 用途 | EIA用標識酵素, 化学発光法によるりん脂質の臨床分析への応用。 | | 用途 | EIA用標準酵素 | | 生物学のな役割 | アルカリホスファターゼ［EC3.1.3.1］．アルカリ性ホスファターゼともいう．アルカリ性領域に至適pHをもつホスホモノエステラーゼ．亜鉛酵素で，特異性の広いホスファターゼ．広く生物の膜画分に存在する．妊娠後期や骨症のときなどに血漿中の濃度が上昇する． | | 使用上の注意 | 安定性安定pH:6～9 | | 化学的特性 | solid | | 使用 | Alkaline Phosphatase, calf intestine, EIA GradeDephosphorylation of cloning vector DNA to prevent recircularization during ligation, dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase, treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis, dephosphorylation of DNA and RNA. It is also used to remove the 5?-terminal phosphate from nucleic acids during molecular cloning reactions. | | 使用 | Hydrolyzes 5′-terminal monophosphate (dephosphorylation) from DNA and RNA. Prevents fragments from self annealing. 5′-nucleic acid targeting for probes. | | 使用 | Alkaline Phosphatase is commonly used to remove the 5′-terminal phosphate from nucleic acids. | | 一般的な説明 | Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase. | | Biochem/physiol Actions | Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA). | | 純化方法 | The E.coli supernatant in sucrose (20%, 33mM) in Tris-HCl pH 8.0 is purified through a DEAE-cellulose column and recrystallised. To the column eluates in 0.125M NaCl is added MgCl2 (to 0.01M) and brought to 50% saturation in (NH4)2SO4 by adding the solid (0.20g/mL). The mixture is centrifuged to remove bubbles and is adjusted to pH 8.0 (with 2N NaOH). Saturated (NH4)2SO4 at pH 8.0 is added dropwise until the solution becomes faintly turbid (~61% saturation). It is set aside at ~25o for 1hour (turbidity will increase). The mixture is placed in an ice bath for several minutes when turbidity disappears and a clear solution is obtained. It is then placed in a large ice bath at 0o (~5L) and allowed to warm slowly to room temperature in a dark room whereby crystals are formed appearing as a silky sheen. The crystals are collected by centrifugation at 25o if necessary. The crystalline solutions are stable at ~25o for many months. They can be stored at 0o, but are unstable when frozen. Cysteine at 10-3M and thioglycolic acid at 10-4M are inhibitory. This is reversed on addition of Zn2+ ions. Many organic phosphates are good substrates for this phosphatase. [Molamy & Horecker Methods Enzymol 9 639 1966, Torriani et al. Methods Enzymol 12b 212 1968, Engstrom Biochim Biophys Acta 92 71 1964.] Alkaline phosphatase from rat osteosarcoma has been purified by Me2CO precipitation and chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxylapatite. [Nair et al. Arch Biochem Biophys 254 18 1987.] Phosphoproteins (various). These are purified by adsorbing onto an iminodiacetic acid substituted agarose column to which are bound ferric ions. This chelate complex acts as a selective immobilised metal affinity adsorbent for phosphoproteins. [Muszyfiska et al. Biochemistry 25 6850 1986.] |
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