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Postion:Product Catalog >Organic Chemistry>Hydrocarbons and derivatives>Hydrocarbon nitrification>5,5′-Dithiobis(2-nitrobenzoic acid)
5,5′-Dithiobis(2-nitrobenzoic acid)
  • 5,5′-Dithiobis(2-nitrobenzoic acid)

5,5′-Dithiobis(2-nitrobenzoic acid) NEW

Price $100 $1
Package 1KG 1000KG
Min. Order: 1KG
Supply Ability: g-kg-tons, free sample is available
Update Time: 2023-12-24

Product Details

Product Name: 5,5′-Dithiobis(2-nitrobenzoic acid) CAS No.: 69-78-3
Min. Order: 1KG Purity: 99%
Supply Ability: g-kg-tons, free sample is available Release date: 2023/12/24
Lead time: In stock Packaging: bottle/ drum/ bucket/ IBC, as request.
Delivery: By sea, by air, by express Origin: Manufacturer

1. Product information

Product Name:5,5′-Dithiobis(2-nitrobenzoic acid)
Synonyms:DTNB;ellmann's reagent;ELLMAN'S REAGENT;ELLMANS' REAGENT;BIS(3-CARBOXY-4-NITROPHENYL) DISULFIDE;3-CARBOXY-4-NITROPHENYL DISULFIDE;Ellmsn'sreagent;Bis(3-carboxy-4-nitrophenyl) disulphide~3-Carboxy-4-nitrophenyl disulphide~Ellmans
CAS:69-78-3
MF:C14H8N2O8S2
MW:396.35
EINECS:200-714-4
Product Categories:marker;Phenyls & Phenyl-Het;Phenyls & Phenyl-Het
Mol File:69-78-3.mol
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5,5′-Dithiobis(2-nitrobenzoic acid) Chemical Properties
Melting point 240-245 °C (dec.)(lit.)
Boiling point 671.9±55.0 °C(Predicted)
density 1.67
refractive index 1.6900 (estimate)
storage temp. Keep in dark place,Sealed in dry,Room Temperature
solubility Do you have solubility information on this product that you would like to share
form Yellow solid
pka1.57±0.25(Predicted)
color Pale Yellow to Light Yellow
Water Solubility Soluble in water and ethanol.
5,5′-Dithiobis(2-nitrobenzoic acid) Usage And Synthesis
Chemical PropertiesCrystalline
Uses5,5′-Dithiobis(2-nitrobenzoic acid) is an membrane-impermeant thiol/disulfide exchange blocker. It is also used to quantify the number or concentration of thiol groups in a sample.
UsesReagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.
Uses5,5′-Dithiobis(2-nitrobenzoic acid) is a water soluble reagent mainly used for the derivatization of sulfhydryl groups.
DefinitionChEBI: An organic disulfide that results from the formal oxidative dimerisation of 2-nitro-5-thiobenzoic acid. An indicator used to quantify the number or concentration of thiol groups.
General Description5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.


2. Packaging

For powders: normal is 25kgs/Drum or bag, or larger/smaller package as request.

For liquids: normal 25kgs/drum, 180-300kgs/bucket, or IBC, determined by the nature of the product. 

                     Or smaller package 1kg/bottle, 10kgs/bottle as request. 


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3. Shipping

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4. Contact information

For more details, pls contact us freely.

Email address : elin@fdachem.com

Mob: 86 13613820652 

WhatsApp/Skype/Wechat/LINE: 86 13613820652

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Company Profile Introduction

Henan Fengda Chemical Co., Ltd. is located in the High-tech Development Zone of Henan Province. Specializing in the production and sales of various fine chemical products required for industrial production, including chemical raw materials, organic raw materials, petrochemicals, chemical reagents, solvents, catalysts, and additives, etc.

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  • Since: 2023-02-10
  • Address: Room 01, 2288 E05, Building 14, East Henan University, Science and Technology Park, 279 Xisanhuan Ro
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