DL-Sulforaphane NEW

Anticancer effect

Sulforaphane is a multifunctional enzyme inducer that can induce the body to produce type II detoxification enzymes glutathione transferase (GST) and quinone reductase (QR), which can neutralize suspected carcinogens and prevent carcinogens from destroying genetic factors in healthy cells, thus playing an anticancer role. Sulforaphane is only one of many isothiocyanate derivatives with anticancer activity. A isothiocyanate compound, whether natural or synthetic, has been found to directly inhibit the methylation of CpGDNA in the gene starter /5-UTR region and also directly inhibit histonedeacethylases. The activity of HADC selectively promotes histone acetylation and methylation, thus restoring the expression of some genes with important functions that are abnormally turned off in cancer cells and giving full play to their normal functions, so that the malignant proliferation of cancer cells can be controlled, which can effectively inhibit colon cancer, stomach cancer, lung cancer, prostate cancer, liver cancer, colon cancer, and so on. Metastatic human tumor cells such as melanoma and neuroblastoma grow. Compared with known DNA methylation inhibitors and natural or synthetic histone deacetylase inhibitors, this series of compounds is a unique dual inhibitor with strong anti-cancer effect, low toxic side effects, and stable biological properties, which can be used to treat and prevent various malignant tumors. Researchers at the UK Food Research Institute believe that sulforaphane can stimulate the body's own anti-cancer mechanisms. Evidence shows that sulforaphane helps antioxidants maintain a healthy balance when the environment, food and other carcinogens affect the body. Because sulforaphane turns on a gene in the body that increases antioxidant levels, it produces an enzyme called HDAC that prevents tumor growth. At the same time, sulforaphane can also promote the human body to produce enzymes for the excretion of carcinogens. When people eat broccoli, they produce sulforaphane because of the ingestion of glycosides. Researchers at the UK Food Research Institute believe that sulforaphane can stimulate the body's own anti-cancer mechanisms. Sulforaphane turns on a human gene that increases levels of antioxidants and blocks an enzyme that promotes tumor growth. Research has shown that Chemicalbook sulfur may halt the development of cancer stem cells, while chemotherapy and radiation therapy may cause cancer cells to grow and rebound.

Extraction and separation

1. Lin Yi et al. extracted sulforaphane from broccoli seeds as raw materials. The results showed that when the hydrolysis pH value was 4.0, the hydrolysis time was 12h, and the extraction solvent was ethyl acetate, the extraction rate of sulforaphane reached 3.5% and the extraction purity exceeded 95%. 2. Liu Xijian et al. studied the separation and purification process of sulforaphane by macroporous resin in order to improve the purity of sulforaphane. The suitable resin for the separation of sulforaphane SP850 was selected by experiments. The results showed that SP850 resin began to leak when treated with about 20BV(1BV=10mL) sulforaphane solution, and the dynamic adsorption curve could be described by Bohart-Adams mathematical model. The optimum process conditions for purification of sulforaphane by SP850 resin are as follows: the upper column liquid volume is 20BV, the upper column flow rate is 5BV/h, the volume fraction of elution ethanol is 50%, the eluent liquid volume is 6BV, and the elution rate is 3BV/h. Under these conditions, the adsorption capacity is 32.83mg/mL resin, and the elution rate is as high as 91.84%. The purity of sulforaphane can reach 88.7%.

Chemical synthesis and enzymatic preparation

At present, there are three main methods to synthesize sulforaphane: 1, chemical synthesis; 2, enzymatic method; 3. Chemical method combined with enzymatic method. 1. Chemical synthesis: In 2003, Desai et al. 's research group synthesized sulforaphane with isotope labeling within five steps, with a yield of ~ 10%. 2, enzymatic method: Extract sulforaphane precursors from cruciferous vegetables - glucosaphane, preparation of sulforaphane by hydrolysis. 3. Combination of chemical method and enzyme method: the combination method of chemical synthesis and enzymatic extraction is to extract glucose-glycerol trerucate from arugula seeds. Glucose-glycerol trerucate is used as raw material to prepare glucose-glucose-glucose-trierucate through selective oxidation reaction, and then sulforaphane is obtained through hydrolysis. This information has been edited by Chemicalbook.

efficacy

It has been studied that sulforaphane has the effect of anti-tumor, help to clear lung bacteria and prevent gout. It can also be used as an adjunct therapy for leukemia.