Procaine NEW
| Price | $44 |
| Package | 500mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-06-12 |
Product Details
| Product Name: Procaine | CAS No.: 59-46-1 |
| Purity: 99.57% | Supply Ability: 10g |
| Release date: 2025/06/12 |
Product Introduction
Bioactivity
| Name | Procaine |
| Description | Procaine (Vitamin H3) is a local anesthetic of the ester type that has a slow onset and a short duration of action. It is mainly used for infiltration anesthesia, peripheral nerve block, and spinal block. |
| Cell Research | For apoptosis analysis, the TUNEL assay is performed on HLE cells treated with no drug(control), or 1 μM of DAC(5-aza-2'-deoxycytidine), or 1 mM of PCA(procaine), or 1 mM of PCAA(procainamide) for 96 h. The proportion of TUNEL-positive cells was calculated by counting at least 500 cells randomly. All assays were carried out in triplicate. (Only for Reference) |
| Kinase Assay | Biochemical Assays: Compound potency is also assessed through incorporation of 3H-SAM into a biotinylated H3 peptide. Specifically, PRC2 containing either EZH1 (160 pM), wt EZH2 (40 pM), or Y641N mutant EZH2 (80 pM, both EZH2 prepared in-house) is pre-incubated with 3H-SAM (0.9 μM), 2 μM H3K27me3 activating peptide (H2N-RKQLATKAAR(Kme3)SAPATGGVKKP-amide) and compounds (as 10 point duplicate dose response titrations) for 120 min in a buffer consisting of 50 mM Tris (pH 8.5), 1 mM DTT, 0.07 mM Brij-35, 0.1% BSA, and 0.8% DMSO in a total volume of 12.5 μl in a black 384 well plate. Reaction is initiated with biotinylated H3 substrate peptides (H3K27me1 for wt EZH2, H3K27me2 for Y641N mutant EZH2; H2N-RKQLATKAAR(Kmen)SAPATGGVKKP-NTPEGBiot) as a 2 μM stock in 12.5 μL and allowed to react at room temperature for 5 h. Quenching is accomplished by addition of 20 μl of STOP solution (50 mM Tris (pH 8.5), 200 mM EDTA, 2 mM SAH). 35 μL of the quenched solution is transferred to Streptavidin Flashplates, incubated overnight, washed, and read in a TopCount Reader. For titrations all compound dilutions are in DMSO, final DMSO concentrations are 0.8% (v/v), and turnover is kept to less than < 5%. IC50s are calculated using non-linear least square four parameter fits (GraphPad 6.0). |
| In vitro | The viability of HLE, HuH7, and HuH6 cells is significantly decreased by procaine treatment. Inhibition of S/G2/M transition, morphological changes such as vacuolation and no increase in apoptosis rate are observed in the procaine-treated HLE cells. All the genes transcriptionally suppressed by DNA hypermethylation are demethylated and reactivated with procaine treatment. Procaine has growth-inhibitory and demethylating effects on human hepatoma cells[2]. |
| In vivo | Procaine has a growth-inhibitory and demethylating effect against xenograft in vivo[2]. |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | H2O : < 1 mg/mL (insoluble or slightly soluble) DMSO : 47 mg/mL (198.89 mM), Sonication is recommended. Ethanol : 43 mg/mL (181.96 mM), Sonication is recommended. |
| Keywords | targets | SodiumChannel | Sodium Channel | RNASynthesis | RNA Synthesis | Procaine | onset | multiple | Inhibitor | inhibit | HistoneDemethylase | Histone Demethylase | duration | DNASynthesis | DNA/RNA Synthesis | DNA Synthesis |
| Inhibitors Related | 5-Fluorouracil | Phenytoin sodium | Rifaximin | Guanidine hydrochloride | Methotrexate disodium | Valproic Acid | N-Nitrosodiethylamine | Lidocaine hydrochloride | L-Aspartic aicd sodium | Cytarabine | Thymidine | Folic acid |
Company Profile Introduction
TargetMol Chemicals Inc. is headquartered in Boston, MA, and specializes in products and services that serve the research needs of chemical and biological scientists worldwide. With a client base in 40+ countries, TargetMol has evolved into one of the biggest global research suppliers for compound libraries and small molecule compounds.
170+ Compound Libraries， 10000+ Noval Small Molecucles，16000+ Nature Compounds
TargetMol diligently updates and offers over 170 types of compound libraries and a wide range of high-quality research chemicals, including inhibitors, activators, natural products, peptides, antibodies , and novel life-science kits for laboratory and scientific use. In addition, our lab allows us to conduct CADD (computer-aided drug design) and chemical synthesis to meet the customization needs of our clients.
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United States- Since: 2011-01-07
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