产品属性:
产品名称 | 规格 | CAS号 | 型号 |
Apoptozole | 10mg 5mg 25mg 50mg | 1054543-47-3 | EY-Y0163842 |
Cas No.1054543-47-3
别名 Apoptosis Activator VII
化学名 4-[[2-[3,5-bis(trifluoromethyl)phenyl]-4,5-bis(4-methoxyphenyl)-1H-imidazol-1-yl]methyl]-benzamide
分子式 C33H25F6N3O3
分子量 625.6
溶解度 ≤10mg/ml in ethanol;50mg/ml in DMSO;50mg/ml in dimethyl formamide
储存条件 Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述:
Apoptozole is an inhibitor of ATPase activity of HSP70 [1].
The heat shock protein 70 (HSP70) protein family plays diverse roles in biological processes and has multiple functions involved in suppression of apoptotic pathways through multiple anti-apoptotic processes. The two major members, constitutive Hsc70 and inducible Hsp70, are composed of an N-terminal ATPase domain and a C-terminal substrate binding domain [1][2].
Apoptozole (Az) is an inhibitor of ATPase activity of HSP70. Apoptozole inhibited the ATPase activity of HSP70 by binding to its ATPase domain. In the presence of 200 μM ATP, Apoptozole inhibited the ATPase activity of HSP70 by 32% and 65% at 100 μM and 200 μM, respectively. Apoptozole bound HSP70 but not HSP40, HSP60, and HSP90. In several cancer cell lines, Apoptozole induced apoptosis in a dose-dependent way with IC50 values ranged from 5 to 7 μM. In HeLa cells, Apoptozole decreased the amount of APAF-1 associated with HSP70 without affecting the expression level of APAF-1, and produced cleaved procaspase-9, suggesting that Apoptozole blocked association of HSP70 with APAF-1 and induced caspase-dependent apoptosis.
In the nude mice xenografted with A549, RKO (colorectal carcinoma), and HeLa cells, i.p injection of Apoptozole reduced tumor volumes by 61%, 65%, and 68%, respectively [1]. In mice administered with both protein antigen and Az, Az increased immune responses to administered antigens and produced more antibodies [2].
关键字: 1054543-47-3;C33H25F6N3O3;Apoptozole;
上海一研生物科技有限公司Shanghai yiyan bio-technology Co. Ltd.主要从事免疫学、分子生物学和常规生化试剂等为一体的科研产品销售企业,公司自成立以来,秉承""全心全意服务于科研工作者""的企业理念,立足生物科技领域,运用生物技术和科研试剂,发展现代生物科技,为各类大中小医院及其它医疗机构、高等院校、科研院所、企事业单位提供优质的产品,服务生物科技领域的科学研究人员。
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