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Journal of periodontal research

Journal of periodontal research

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Impaired autophagy flux by lncRNA NEAT1 is critical for inflammation factors production in human periodontal ligament stem cells with nicotine treatment

Published:8 November 2022 DOI: PMID: 36346119
Taotao Zhang, Kuan Yang, Yujiang Chen, Yuran Jiang, Zhifei Zhou, Jiajia Liu, Yang Du, Lulu Wang, Xinxin Han, Xingan Wu, Xiaojing Wang

Abstract

Background and Objectives

Periodontitis is the top reason for tooth loss, and smoking significantly increases severe periodontitis risk. Defective autophagy has been reported to play a vital role in periodontitis. This study aimed to elucidate the relationship between autophagy and inflammation factors production in nicotine-treated periodontal ligament stem cells (PDLSCs) and the underlying mechanism.

Methods

In this study, transmission electron microscopy, immunofluorescence, and the mCherry-GFP-LC3 plasmid were used to study autophagy flux. The gene levels of inflammation factors and long noncoding RNA nuclear paraspeckle assembly transcript 1 (lncRNA NEAT1) were detected by quantitative real-time PCR (qRT-PCR). Western blot was performed to assess the protein levels of autophagic markers and α7 nicotinic acetylcholine receptor (α7nAChR).

Results

We found that nicotine impaired autophagosome-lysosome fusion and lysosome functions to block autophagy flux, contributing to inflammatory factors production in nicotine-treated PDLSCs. Moreover, nicotine upregulated NEAT1 by activating α7nAChR. NEAT1 decreased autophagy flux by downregulating syntaxin 17 (STX17).

Conclusion

Our data indicate that NEAT1-decreased autophagy flux is pivotal for inflammation factors production in nicotine-treated PDLSCs.

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Materials
Procduct Name CAS Molecular Formula Supplier Price
N-(3R)-1-AZABICYCLO[2.2.2]OCT-3-YL-4-CHLOROBENZAMIDE 123464-89-1 C14H17ClN2O 160 suppliers $9.00-$5653.73
N-(3R)-1-AZABICYCLO[2.2.2]OCT-3-YL-4-CHLOROBENZAMIDE 123464-89-1 C14H17ClN2O 160 suppliers $9.00-$5653.73
N-(3R)-1-AZABICYCLO[2.2.2]OCT-3-YL-4-CHLOROBENZAMIDE 123464-89-1 C14H17ClN2O 160 suppliers $9.00-$5653.73
N-(3R)-1-AZABICYCLO[2.2.2]OCT-3-YL-4-CHLOROBENZAMIDE 123464-89-1 C14H17ClN2O 160 suppliers $9.00-$5653.73

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