Laccase-catalyzed tyrosine click reaction with 1-methyl-4-arylurazole: rapid labeling on protein surfaces

Published:5 November 2024 DOI: 10.1039/D4CC03802A

Keita Nakane , Chizu Fujimura , Shogo Miyano , Zhengyi Liu , Tatsuya Niwa , Hafumi Nishi , Tetsuya Kadonosono , Hideki Taguchi , Shusuke Tomoshige , Minoru Ishikawa , Shinichi Sato

Abstract

Our study demonstrates the exceptional efficiency of 1-methyl-4-arylurazole (MAUra) for tyrosine labeling, optimized with laccase under mild conditions, achieving a high efficiency (k_cat/K_m = 7.88 × 10⁴ M⁻¹ s⁻¹) with minimal oxidative side reactions and selective labeling of highly exposed tyrosine sites on proteins.