Assembly of Fe3O4@SiO2 Magnetic Nanochains and Application of Glucose Oxidase Immobilized by Nanochains in Flow Injection Chemiluminescence Systems

Abstract

Fe3O4 magnetic nanoparticles, as typical magnetic nanomaterials, can be functionalized and self-assembled into one-dimensional and two-dimensional structures to obtain new physicochemical properties. However, there are few studies on preparing stable large-sized one-dimensional Fe3O4 magnetic nanochains structure by a simple self-assembly method, and its application scope needs to be expanded urgently. In this paper, a large-size Fe3O4@SiO2 one-dimensional nanochains were synthesized and modified by means of different functionalization. On this basis, the application of one-dimensional nanochains in the flow injection chemiluminescence analysis was explored. Glucose oxidase was immobilized on the Fe3O4@SiO2 nanochains, which as a packed stationary phase, was successfully coupled with the flow injection system through the optimization of experimental conditions. As a result, a novel flow injection chemiluminescence analysis of glucose was developed. It has been proven that the one-dimensional nanochains-based packed stationary phase not only retains similar catalytic sites as the free magnetic particles, but also effectively overcomes the problem of poor fluidity of the free fixed phase. Therefore, it is expected to explore more high-performance flow injection analysis systems by strategies of the nanochain-immobilized enzyme-based packed stationary phase.