4,5-dihydroxy-2-oxo-6-phosphonooxy-hexanoic acid synthesis
- Product Name:4,5-dihydroxy-2-oxo-6-phosphonooxy-hexanoic acid
- CAS Number:27244-54-8
- Molecular formula:C6H11O9P
- Molecular Weight:258.12
17510-99-5
17 suppliers
$325.00/5mg
27244-54-8
12 suppliers
$95.00/5mg
Yield:-
Reaction Conditions:
with Saccharophagus degradans 2-40T 2-keto-3-deoxy-D-gluconate kinase;ATP in aq. buffer at 30; for 1 h;Enzymatic reaction;
Steps:
General procedure: To test enzyme activities of KDG kinase and KDPG aldolase in vitro, 800μg/mL of each purified enzyme were sequentially added to post-reaction mixtures that contained the corresponding substrates for the target enzymes. For the reaction by KDG kinase, 2.5mM of ATP was added in the reaction mixture as a phosphoryl donor. After incubating at 30°C for 1h, each enzymatic reaction mixture was quenched in boiling water for 5min. The production of KDPG by putative KDG kinase was confirmed by TLC and GC/MS, and the production of pyruvate by putative KDPG aldolase was confirmed by gas chromatography/time-of-flight mass spectrometry (GC/TOF MS).
References:
Kim, Do Hyoung;Wang, Damao;Yun, Eun Ju;Kim, Sooah;Kim, Soo Rin;Kim, Kyoung Heon [Process Biochemistry,2016,vol. 51,# 10,p. 1374 - 1379]
591-57-1
15 suppliers
$376.00/1ml
127-17-3
567 suppliers
$5.00/10g
27244-54-8
12 suppliers
$95.00/5mg