ChemicalBook CAS DataBase List H-SER-TYR-OH

H-SER-TYR-OH synthesis

6synthesis methods

Product Name:H-SER-TYR-OH
CAS Number:21435-27-8
Molecular formula:C12H16N2O5
Molecular Weight:268.27

201855-53-0
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21435-27-8
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Yield:-

Reaction Conditions:

with ethylenediaminetetraacetic acid;dipeptydyl-peptidase DPP₅;sodium chloride in aq. phosphate buffer at 37; pH=6.7; for 0.5 h;Catalytic behavior;Kinetics;Enzymatic reaction;Reagent/catalyst;pH-value;

Steps:

Hydrolyzing Activity toward Dipeptidyl MCA

General procedure: Hydrolyzing Activity toward Dipeptidyl MCA-Recombinantpeptidases (1-50 ng) were incubated with 20 M dipeptidyl-MCA in 200 l of reaction solution composed of 50 mMsodium phosphate (pH 7.0), 5 mM EDTA, and 0.1 M NaCl at37 °C for 30 min. In several experiments,pHwas varied using 50mM sodium phosphate buffer and the NaCl concentrationswere varied from 0 to 1.6 M. Fluorescence intensity was measuredwith excitation at 380 nm and emission at 460 nm with aFluorescence Photometer F-4000 (Hitachi, Tokyo, Japan). Todetermine the enzymatic parameters, recombinant proteins (5ng) were incubated with various concentrations of dipeptidyl-MCA (0.5-160 M) in the presence of 0.1 M NaCl at pH 6.7.Data were analyzed using a nonlinear regression curve fitted tothe Michaelis-Menten equation using the GraphPad Prismsoftware program (San Diego, CA). Averages and S.E. were calculatedfrom 4 independent measurements

References:

Ohara-Nemoto, Yuko;Rouf, Shakh M. A.;Naito, Mariko;Yanase, Amie;Tetsuo, Fumi;Ono, Toshio;Kobayakawa, Takeshi;Shimoyama, Yu;Kimura, Shigenobu;Nakayama, Koji;Saiki, Keitarou;Konishi, Kiyoshi;Nemoto, Takayuki K. [Journal of Biological Chemistry,2014,vol. 289,# 9,p. 5436 - 5448]