| Identification | More | [Name]
2-Iodoacetamide | [CAS]
144-48-9 | [Synonyms]
2-IODOACETAMIDE
ALPHA-IODOACETAMIDE
IODOACETAMIDE
2-iodo-acetamid
Acetamide, 2-iodo-
Monoiodoacetamide
Surauto
USAF d-1
usafd-1
0IODOACETAMIDE CRYSTALLINE
ALKYLATING REAGENT, IODOACETAMIDE
IODOACETAMIDE SIGMAULTRA
2-IODOACETAMIDE,CRYSTAL
IODACETAMIDE
2-Iodoacetamide, 98%, stab. with ca 5-8% water
Iodoethanamide
2-Iodoacetamide [for Biochemical Research] | [EINECS(EC#)]
205-630-1 | [Molecular Formula]
C2H4INO | [MDL Number]
MFCD00008028 | [Molecular Weight]
184.96 | [MOL File]
144-48-9.mol |
| Chemical Properties | Back Directory | [Appearance]
yellow-brown crystals | [Melting point ]
92-95 °C(lit.)
| [Boiling point ]
297.1±23.0 °C(Predicted) | [density ]
2.1103 (estimate) | [storage temp. ]
2-8°C
| [solubility ]
H2O: 0.5 M at 20 °C, clear, colorless
| [form ]
crystalline
| [pka]
15.16±0.40(Predicted) | [color ]
White to pale yellow or beige | [Stability:]
Stable, but light sensitive. Incompatible with strong oxidizing agents, strong bases, reducing agents, acids. | [Water Solubility ]
slightly soluble | [Sensitive ]
Light Sensitive | [BRN ]
1739080 | [InChIKey]
PGLTVOMIXTUURA-UHFFFAOYSA-N | [CAS DataBase Reference]
144-48-9(CAS DataBase Reference) | [NIST Chemistry Reference]
Alpha-iodo acetamide(144-48-9) | [EPA Substance Registry System]
144-48-9(EPA Substance) |
| Safety Data | Back Directory | [Hazard Codes ]
T | [Risk Statements ]
R25:Toxic if swallowed.
R42/43:May cause sensitization by inhalation and skin contact .
R43:May cause sensitization by skin contact.
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S22:Do not breathe dust .
S36/37:Wear suitable protective clothing and gloves .
S45:In case of accident or if you feel unwell, seek medical advice immediately (show label where possible) .
S37/39:Wear suitable gloves and eye/face protection .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S24:Avoid contact with skin . | [RIDADR ]
UN 2811 6.1/PG 3 | [WGK Germany ]
3 | [RTECS ]
AC4200000 | [F ]
8-10-21 | [TSCA ]
T | [HazardClass ]
6.1 | [PackingGroup ]
III | [HS Code ]
29241900 |
| Hazard Information | Back Directory | [Chemical Properties]
yellow-brown crystals | [Uses]
Alkylating agent used in peptide mapping because it covalently binds with the thiols in cysteine to prevent disulfide bond formation2-Iodoacetamide is used as an electrophile for covalent modification of nucleophilic residues on proteins such as cysteine, methionine and histidine. It is used to bind with thiol group of cysteine, thereby it protects the formation disulfide bonds. It is involved as an inhibitor of deubiquitinase enzymes (DUBs). Further, it acts as an alkylating sulfhydryl reagent. | [Application]
Alkylating agent used in peptide mapping because it covalently binds with the thiols in cysteine to prevent disulfide bond formation 2-Iodoacetamide is used as an electrophile for covalent modification of nucleophilic residues on proteins such as cysteine, methionine and histidine. It is used to bind with thiol group of cysteine, thereby it protects the formation disulfide bonds. It is involved as an inhibitor of deubiquitinase enzymes (DUBs). Further, it acts as an alkylating sulfhydryl reagent. | [Synthesis]
2-Iodoacetamide is synthesized by reacting chloroacetamide with sodium iodide. The chloroacetamide, anhydrous acetone, and anhydrous sodium iodide were refluxed on the bath for 15h. Cool to room temperature, filter out sodium chloride, recover acetone, pour into ice water of sodium bisulfate after a little cooling, and then neutralize to pH 6 with saturated sodium sulfate solution. Cool to crystallize and filter to obtain crude product. The crude product is recrystallized with water to obtain the finished product. | [Purification Methods]
Crystallise it from water or CCl4. It is used for tagging proteins. [Gurd Methods Enzymol 25 424 1972, Beilstein 2 IV 536.] | [Preparation and handling]
Alkylation Procedure
Iodoacetamide is unstable and light-sensitive. Prepare solutions immediately before use and perform alkylation in the dark. If iodoacetamide is present in limiting quantities and a slightly alkaline pH, cysteine modification will be the exclusive reaction. Excess iodoacetamide or non-buffered iodoacetamide reagent can also alkylate amines (lysine, N-termini), thioethers (methionine), imidazoles (histidine) and carboxylates (aspartate, glutamate).
1. Add 5 μl of 2% SDS and 45 μl of 200 mM ammonium bicarbonate (pH 8.0) to 20-100 μg of protein sample. Adjust volume to 100 μl with ultrapure water.
2. Add 5 μl of 200 mM Tris(2-carboxyethyl) phosphine hydrochloride (TCEP.HCl, Product No. 20490) and incubate sample at 55°C for 1 hour.
3. Immediately before use, dissolve one tube of iodoacetamide (9.3 mg) with 132 μl of 200 mM ammonium bicarbonate (pH 8.0) to make 375 mM iodoacetamide. Protect solution from light.
4. Add 5 μl of the 375 mM iodoacetamide to the sample and incubate for 30 minutes protected from light.
5. Proceed to proteolytic digestion before MS analysis or other processing. | [Precautions]
Store in a cool place. Light and moisture sensitive. Keep the container tightly closed in a dry and well-ventilated place. Incompatible with strong acids, strong bases, strong oxidizing agents and strong reducing agents. |
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