| Identification | More | [Name]
Guanidine thiocyanate | [CAS]
593-84-0 | [Synonyms]
AMINOMETHANAMIDINE THIOCYANATE
GTC
GUANIDINE HYDROTHIOCYANATE
GUANIDINE ISOTHIOCYANATE
GUANIDINE MONOTHIOCYANATE
GUANIDINE RHODANIDE
GUANIDINE THIOCYANATE
GUANIDINIUM ISOTHIOCYANATE
GUANIDINIUM RHODANIDE
GUANIDINIUM THIOCYANATE
Thiocyanicacid,compd.withguanidine(1:1)
usafek-705
Guandine thiocyanate
GUANIDINE THIOCYANATE SOLUTION, F. MOL. BIOLOGY, 4 M IN H2O
GUANIDINE THIOCYANATE, 4X10 G*
GUANIDINE THIOCYANATE MOLECULAR BIOLOGY REAGENT
GUANIDINE THIOCYANATE SOLUTION, F. MOL. BIOLOGY, 6 M IN H2O
GUANIDINE THIOCYANATE, FOR MOLECULAR BIO LOGY
GUANIDINE THIOCYANATE SIGMAULTRA
GUANIDINE THIOCYANATE 99+% | [EINECS(EC#)]
209-812-1 | [Molecular Formula]
C2H4N4S | [MDL Number]
MFCD00013027 | [Molecular Weight]
116.14 | [MOL File]
593-84-0.mol |
| Chemical Properties | Back Directory | [Appearance]
White cryst.powder | [Melting point ]
120-122 °C(lit.)
| [bulk density]
630kg/m3 | [density ]
1.103 g/mL at 20 °C
| [vapor pressure ]
0Pa at 20℃ | [refractive index ]
n20/D 1.482
| [storage temp. ]
Store at RT. | [solubility ]
H2O: 6 M at 20 °C, clear, colorless
| [form ]
Crystals or Crystalline Powder | [pka]
12.5[at 20 ℃] | [color ]
White or colorless | [Specific Gravity]
1.152 | [Odor]
Odorless | [PH]
4.8-6.0 (1420g/l, H2O, 20℃)(saturated solution) | [PH Range]
4.8 - 6.0 | [Stability:]
Stability Stable, but light sensitive. Incompatible with acids (contact releases very toxic gas), strong oxidising agents. | [biological source]
synthetic (organic) | [Water Solubility ]
Soluble in water and ethanol | [Sensitive ]
Light Sensitive | [Detection Methods]
T | [BRN ]
3563461 | [InChI]
1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H | [InChIKey]
ZJYYHGLJYGJLLN-UHFFFAOYSA-N | [SMILES]
SC#N.NC(N)=N | [LogP]
-1.5--1.42 at 20℃ | [Uses]
Potent protein denaturant used in isolation of intact DNA, RNA. | [CAS DataBase Reference]
593-84-0(CAS DataBase Reference) | [Storage Precautions]
Light sensitive | [EPA Substance Registry System]
593-84-0(EPA Substance) | [Absorption]
cut-off at 280nm in H2O at 6M |
| Questions And Answer | Back Directory | [Preparation]
method for preparing guanidine thiocyanate, comprising the following process flow: (1) Guanidine carbonate and ammonium sulfate in a weight ratio of 2:1 are placed in a reaction vessel and reacted at 45-95℃ for 1.5 hours to obtain crude guanidine thiocyanate. (2) Dissolution: At room temperature, the crude guanidine thiocyanate is dissolved in water at a ratio of 0.5:1. (3) Filtration: Impurities such as raw materials and reaction by-products are removed by filtration. (4) Dehydration: The mother liquor after filtration is dehydrated at high temperature. (5) Crystallization: The solution is cooled, concentrated, and crystallized to obtain high-purity guanidine thiocyanate. | [Description]
Guanidine thiocyanate is one of the cheapest and easiest to prepare of the guanidine salts. Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent. It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents. It is provided in one bottle containing 500 g. It is recommended for isolation of RNA, especially for tissues such as pancreas with high levels of RNase activity. In solution, it is known as guanidinium thiocyanate. Guanidine Thiocyanate is thoroughly tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity. | [Uses]
Guanidine thiocyanate is a potent protein denaturant (stronger than guanidine HCl) often used in the isolation of intact ribonucleic acid to eliminate RNase activity. RNase can recover activity after boiling, but is irreversibly inactivated in a 4 M solution of guanidine thiocyanate. Such solutions, to which the reducing agent b-mercaptoethanol is often added, are used to inactivate RNAse when isolating RNA from tissues that are rich in RNase, such as liver. Total nuclear and cytoplasmic RNA may be isolated this way. A protocol for extracting RNA with guanidine isothiocyanate has been published.
In the presence of guanidine thiocyanate, proteins dissolve readily, cellular structures disintegrate and nucleoproteins dissociate from nucleic acids, as protein secondary structure is lost. | [Biochem/physiol Actions]
Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol−chloroform in RNA extraction. | [Toxicity evaluation]
Guanidine thiocyanate is used commercially as a disinfectant and a general protein denaturant but its more common use is in the extraction of DNA and RNA in molecular biology. Нere are no reports of guanidine thiocyanate toxicity from oral ingestion. Toxicity of diوٴerent thiocyanate compounds other than guanidine thiocyanate has been described. Potassium thiocyanate was used over 50 years ago for the treatment of hypertension and its toxicity appears to cause a neurological disorder consisting of generalized weakness, delirium and a decrease level of consciousness. Reports of thiocyanate toxicity from nitroprusside use for hypertension in the setting of renal impairment/failure have also been reported as thiocyanate is renally cleared. In a fatal deliberate ingestion of a herbicide which contained ammonium thiocyanate and aminotriazole, a 54 year old man presented with coma and cardiovascular collapse although the relative contributions of the two herbicides to the clinical toxicity is unknown. |
| Safety Data | Back Directory | [Symbol(GHS) ]
 
GHS05,GHS07 | [Signal word ]
Danger | [Hazard statements ]
H302+H312+H332-H314-H412 | [Precautionary statements ]
P260-P273-P280-P303+P361+P353-P304+P340+P310-P305+P351+P338 | [Hazard Codes ]
Xn,T | [Risk Statements ]
R20/21/22:Harmful by inhalation, in contact with skin and if swallowed .
R32:Contact with acids liberates very toxic gas.
R52/53:Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment .
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S36/37:Wear suitable protective clothing and gloves .
S61:Avoid release to the environment. Refer to special instructions safety data sheet .
S13:Keep away from food, drink and animal feeding stuffs .
S36:Wear suitable protective clothing .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . | [RIDADR ]
1759 | [WGK Germany ]
2
| [RTECS ]
XL1225000
| [F ]
3-10 | [TSCA ]
Yes | [REACH Registrations]
Active | [HazardClass ]
8 | [PackingGroup ]
III | [HS Code ]
29252000 | [Storage Class]
8B - Non-combustible corrosive hazardous materials | [Hazard Classifications]
Acute Tox. 4 Dermal
Acute Tox. 4 Inhalation
Acute Tox. 4 Oral
Aquatic Chronic 3
Eye Dam. 1
Skin Corr. 1C |
| Hazard Information | Back Directory | [Hazard]
Irritant. | [Chemical Properties]
White cryst.powder | [Application]
Guanidine thiocyanate is a chaotropic agent, protein denaturant and protector of DNA and RNA. The thiocyanate has an enzyme (nuclease) inhibiting function which, combined with its ability to protect nucleic acids, makes it useful in the study of DNA and RNA. It can be used to deactivate viruses so that they can be studied safely. It is used in PCR, Northern and Southern blotting. | [General Description]
Guanidine thiocyanate is chaotropic salts which lyse cells and along with nuclease binding matrix it is also seen to inhibit nuclease activity during extraction of DNA. It works as a strong RNase inhibitor in extraction buffers. | [Flammability and Explosibility]
Notclassified |
|
| Company Name: |
J & K SCIENTIFIC LTD.
|
| Telephone: |
18210857532 18210857532 |
| Website: |
https://www.jkchemical.com |
| Company Name: |
Alfa Aesar
|
| Telephone: |
400-6106006 |
| Website: |
http://chemicals.thermofisher.cn |
| Company Name: |
Energy Chemical
|
| Telephone: |
400-0056266 |
| Website: |
www.energy-chemical.com |
| Company Name: |
Jia Xing Isenchem Co.,Ltd
|
| Telephone: |
0573-85285100 18627885956 |
| Website: |
https://www.chemicalbook.com/ShowSupplierProductsList14265/0_EN.htm |
|