| Identification | Back Directory | [Name]
H-ARG-ARG-LEU-ILE-GLU-ASP-ASN-GLU-TYR-THR-ALA-ARG-GLY-OH | [CAS]
81493-98-3 | [Synonyms]
Tyk
RRLIEDNEYTARG
ZAP-70 (pan Tyr)
PP60V-SRC FRAGMENT
408-420-Src kinase
TYROSINE KINASE SUBSTRATE
PP60(V-SRC) AUTOPHOSPHORYLATION SITE
408-420-Src kinase (synthetic fragment)
TYROSINE KINASE SUBSTRATE SYNTHETIC >96%
arg-arg-leu-ile-glu-asp-asn-glu-tyr-*thr-ala-arg-
pp60(v-SRC) Autophosphorylation Site,RRLIEDNEYTARG
ARG-ARG-LEU-ILE-GLU-ASP-ASN-GLU-TYR-THR- ALA-ARG-GL
ARG-ARG-LEU-ILE-GLU-ASP-ASN-GLU-TYR-THR-ALA-ARG-GLY
H-ARG-ARG-LEU-ILE-GLU-ASP-ASN-GLU-TYR-THR-ALA-ARG-GLY-OH
pp60v-src Autophosphorylation Site, Tyrosine Kinase Substrate
H-ARG-ARG-LEU-ILE-GLU-ASP-ASN-GLU-TYR-THR-ALA-ARG-GLY-OH USP/EP/BP
pp60(v-SRC) Autophosphorylation Site, Protein Tyrosine Kinase Substrate
Glycine,L-arginyl-L-arginyl-L-leucyl-L-isoleucyl-L-a-glutamyl-L-a-aspartyl-L-asparaginyl-L-a-glutamyl-L-tyrosyl-L-threonyl-L-alanyl-L-arginyl-
Glycine, L-arginyl-L-arginyl-L-leucyl-L-isoleucyl-L-α-glutamyl-L-α-aspartyl-L-asparaginyl-L-α-glutamyl-L-tyrosyl-L-threonyl-L-alanyl-L-arginyl- | [Molecular Formula]
C66H109N23O23 | [MDL Number]
MFCD00076728 | [MOL File]
81493-98-3.mol | [Molecular Weight]
1592.71 |
| Chemical Properties | Back Directory | [density ]
1.53±0.1 g/cm3(Predicted) | [storage temp. ]
−20°C
| [form ]
Solid | [pka]
3.31±0.10(Predicted) | [color ]
White to off-white | [Sequence]
Arg-Arg-Leu-Ile-Glu-Asp-Asn-Glu-Tyr-Thr-Ala-Arg-Gly | [CAS DataBase Reference]
81493-98-3 |
| Hazard Information | Back Directory | [Uses]
Residues 2-12 are the v-Src (412-422) autophosphorylation site with an extra Arg for phosphocellulose binding. This substrate sequence, RRLIEDNEYTARG, was originally described as an EGFR substrate in A431 cell extracts (Km=0.3-0.6 mM). | [References]
[1] Ohguro H, et al. Separation of phospho- and non-phosphopeptides using reverse phase column chromatography. FEBS Lett. 1995 Jul 24;368(3):452-4. DOI:10.1016/0014-5793(95)00710-q
[2] Ernould AP, et al. Purification and characterization of the major tyrosine protein kinase from the human promyelocytic cell line, HL60. Eur J Biochem. 1993 Jun 1;214(2):503-14. DOI:10.1111/j.1432-1033.1993.tb17948.x
[3] L J Pike, B Gallis, et al. Epidermal growth factor stimulates the phosphorylation of synthetic tyrosine-containing peptides by A431 cell membranes. Proc Natl Acad Sci U S A. 1982 Mar;79(5):1443-7. DOI:10.1073/pnas.79.5.1443 |
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