480-44-4
480-44-4 结构式
基本信息
刺槐黄素
刺槐素
5,7-二羥-4'-甲氧黃酮
阿卡西汀
洋槐素
5,7-二羟基-4'-甲氧基黄酮
ACACETIN
LINARIGENIN
METHYL-4'-APIGENIN
5,7-dihydroxy-2-(4-methoxyphenyl)-4h-1-benzopyran-4-on
5,7-dihydroxy-4’-methoxy-flavon
5,7-dioxy-4’-methoxyflavone
acacetine
apisenin4’-methylether
buddleoflavonol
5,7-dihydroxy-2-(4-methoxyphenyl)-4-benzopyrone
Abietic
ACACETIN(METHYL-4'-APIGENIN)(RG)
5,7-DIHYDROXY-2-(4-METHOXYPHENOL)-1-BENZOPYRAN-4-ONE
1-BENZOPYRAN-4-ONE,5,7-DIHYDROXY-2-(4-METHOXYPHENOL)-
ACACETIN hplc
ACACETIN WITH HPLC
4'-O-Methylapigenin
5,7-Dihydroxy-2-(4-methoxyphenyl)-4H-1-benzopyran-4-one
Linarisenin
物理化学性质
常见问题列表
金合欢 （Acacia farnesiana (Linn.) Willd.） (Mimosa) 豆科。灌木，高2-4米；枝具刺，刺长可达1-2厘米。二回羽状复叶，羽片4-8对，每羽片具小叶10-20对，小叶片线状长椭圆形。头状花序腋生，直径1.5厘米，常多个簇生。荚果圆柱形，长3-7厘米，直径8-15毫米。种子多数，黑色。 花极香，供提取香精。 刺灌术或小乔木，二回羽状复叶，头状花序簇生于叶腋，盛开时，好像金色的绒球一般。
Acacetin (5,7-Dihydroxy-4'-methoxyflavone; 10-200 μM; 24 hours) decreases cell viabilities in a dose-dependent manner. Acacetin has little effect on human normal glial cell line HEB and non-tumorigenic epithelial cell line MCF-10A.
Acacetin (50-150 μM; 24 hours) causes G2/M cell cycle arrest and induces apoptosis and autophagy.
Acacetin (50-150 μM; 24 hours) leads to decreases in levels of PI3Kγ-p110, p-AKT, p-mTOR, p-p70S6K, and p-ULK in a dose-dependent manner.
Cell Viability Assay
| Cell Line: | Breast cancer MCF-7 cells, hepatocellular carcinoma SMMC-7721 cells, lung adenocarcinoma A549 cells, esophageal carcinoma Eca109 cells |
| Concentration: | 10, 20, 40, 60, 80, 100, 150, 200 μM |
| Incubation Time: | 24 hours |
| Result: |
Decreased cancer cell viabilities in a dose-dependent manner.
Had IC 50 values of 82.75 μM, 103.9 μM, 157.4 μM, 54.7 μM in MDA-MB-231, MCF-7, A549, Eca109 cells, respectively. |
Cell Cycle Analysis
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 50, 100, 150 μM |
| Incubation Time: | 24 hours |
| Result: | Resulted in increase in percentage of cells at G2/M phase and decrease in percentage of cells at G1 and S phase in a dose-dependent manner. |
Apoptosis Analysis
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 50, 100, 150 μM |
| Incubation Time: | 24 hours |
| Result: | Induced apoptosis. |
Cell Autophagy Assay
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 50, 100, 150 μM |
| Incubation Time: | 24 hours |
| Result: |
Induced autophagy.
Resulted in marked increases in EGFP-LC3 puncta formation and a dose-dependent accumulation of LC3-II. |
Western Blot Analysis
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 50, 100, 150 μM |
| Incubation Time: | 24 hours |
| Result: |
Resulted in decrease in levels of Bcl-2 and Bcl-xL and increase in levels of p53.
Led to decreases in levels of PI3Kγ-p110, p-AKT, p-mTOR, p-p70S6K, and p-ULK in a dose-dependent manner. Had little or no effect on expression of PI3Kα, PI3Kβ, PI3Kδ, p-ERK, p-p38, and p-JNK. |
Acacetin (5,7-Dihydroxy-4'-methoxyflavone; 5, 20 mg/kg/day; orally; for 3 days) significantly suppresses microglial activation in an LPS-induced neuroinflammation mouse model.
Acacetin (25 mg/kg/day; orally; for 3 days) reduces neuronal cell death in an animal model of ischemia.
Acacetin (1.8-56.2 mg/kg/day; ip; single dose) decreases visceral and inflammatory nociception and prevented the formalin-induced oedema.
| Animal Model: | Male C57BL/6 mice, 7 weeks of age |
| Dosage: | 5, 20 mg/kg |
| Administration: | Orally; once a day for 3 days |
| Result: | Significantly suppressed microglial activation in an LPS-induced (ip; 5mg/kg) neuroinflammation mouse model. |