64987-85-5
中文名称
4-(N-马来酰亚胺基甲基)环己烷-1-羧酸琥珀酰亚胺酯
英文名称
N-Succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate
CAS
64987-85-5
分子式
C16H18N2O6
MDL 编号
MFCD00009634
分子量
334.32
MOL 文件
64987-85-5.mol
更新日期
2024/04/19 16:05:23
64987-85-5 结构式
基本信息
中文别名
4-(N-马来酰亚胺基甲基)环己烷-1-羧酸琥珀酰亚胺酯 英文别名
4-(N-MALEIMIDOMETHYL)CYCLOHEXANE-1-CARBOXYLIC ACID N-HYDROXYSUCCINIMIDE ESTER4-(N-MALEIMIDOMETHYL)CYCLOHEXANECARBOXYLIC ACID N-HYDROXYSUCCINIMIDE ESTER
N-SUCCINIMIDYL 4-(MALEIMIDOMETHYL)CYCLOHEXANE-1-CARBOXYLATE
N-SUCCINIMIDYL 4-(MALEIMIDOMETHYL)CYCLOHEXANECARBOXYLATE
SMCC
SUCCINIMIDYL 4-[N-MALEIMIDO-METHYL]CYCLOHEXANE-1-CARBOXYLATE
SUCCINIMIDYL TRANS-4-(MALEIMIDYLMETHYL)CYCLOHEXANE-1-CARBOXYLATE
SUCCINIMIDYL-TRANS-4-(N-MALEIMIDOMETHYL)CYCLOHEXANE-1-CARBOXYLATE
SUCCINIMIDYL-TRANS-4-(N-MALEIMIDYLMETHYL)CYCLOHEXANE-1-CARBOXYLATE
TRANS-4-(N-MALEIMIDYLMETHYL)-CYCLO-HEXANE-1-CARBOXYLATE-N-HYDROXYSUCCINIMIDE ESTER
n-(4-carboxycyclohexylmethyl)maleimiden-hydroxysuccinimideester
N-succinimidyl 4-(maleimidome.)cyclo-hexanecarboxylate
4-(N-MALEIMIDOMETHYL)-CYCLOHEXANE-1-CARBOXYLIC ACID N-HYDROXYSUCCINIMIDE ESTER CRYSTALLINE
N-Succinimidyl-4-(N-maleimidomethyl)-cyclohexane-1-carboxylate
4-(maleimidomethyl)-1-cyclohexanecarboxylic acid N-hydroxy
N-Succinimidyl 4-(maleimidomethyl)cyclohexanecarboxylate, SMCC
所属类别
有机原料：羧酸类化合物及衍生物物理化学性质
熔点180-182 °C(lit.)
沸点501.7±42.0 °C(Predicted)
密度1.42±0.1 g/cm3(Predicted)
储存条件2-8°C
溶解度chloroform: 50 mg/mL
酸度系数(pKa)-2.24±0.20(Predicted)
形态powder
颜色白色至灰白色
敏感性Moisture & Light Sensitive
BRN1555271
InChIInChI=1S/C16H18N2O6/c19-12-5-6-13(20)17(12)9-10-1-3-11(4-2-10)16(23)24-18-14(21)7-8-15(18)22/h5-6,10-11H,1-4,7-9H2
InChIKeyJJAHTWIKCUJRDK-UHFFFAOYSA-N
SMILESC1(C(ON2C(=O)CCC2=O)=O)CCC(CN2C(=O)C=CC2=O)CC1
应用领域
用途1
单克隆的SMCC抗体衍生化与低(聚)核苷酸结合，用于两步放射免疫疗法中；双异官能团交联试剂，将胺以及巯基反应性合并入扩展间隔片，典型地，在pH7.5缓冲液(6.5-8.5)中，通过酰胺键偶联至包含伯胺的分子，在pH6.8缓冲液(6.5-7.0)中，通过硫醚连接，次级偶联至特别的包含自由巯基的分子；用于制备酶的免疫交联物以及半抗原载体分子结合体，包含了9个原子连接；一种扩展的脂肪质的间隔片，与芳香族间隔片相比，使顺丁烯二酰亚胺稳定地优先偶联用途2
A sulfhydryl and amino reactive heterobifunctional protein crosslinking reagent. It conjugates anti-digoxin F(ab’2 fragments to 脽-galactosidase. Conjugates hIgG to alkaline phosphatase.常见问题列表
生物活性
SMCC 是一种蛋白质交联剂。SMCC 接合抗原耦合脾脏细胞来诱导抗原特异性免疫反应。体外研究
SMCC or sulfo-SMCC can use to conjugate keyhole limpet hemocyanin (KLH) and ovalbumin (OVA). In the the conjugation system, 300 μl KLH or OVA (3 mg) and 30 μl SMCC or Sulfo-SMCC (the final concentration of 0.1 mM) are added in PBS with final volume of 3 ml.
体内研究
Using the SMCC-conjugation method is able to induce potent immune responses to whole protein or peptide antigens by injection of antigen-coupled syngeneic splenic mononuclear cells in mice.