Calpain Substrate III, Fluorogenic - Calbiochem Chemische Eigenschaften,Einsatz,Produktion Methoden
Allgemeine Beschreibung
A fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 based on 106 known cleavage sites. It is cleaved by mammalian calpains with a much better efficacy (k
cat/K
mx10
-2 (M
-1s
-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and <0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively) and kinetically superior to other commonly used calpain substrates, such as LY-AMC and α-spectrin cleavage site-based substrate. Has been used successfully in monitoring calpain activity in whole Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.
Biochem/physiol Actions
Product does not compete with ATP.
Calpain Substrate III, Fluorogenic - Calbiochem Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
