AXIS Axon Isolation Device, 150 μm

CAS No.
Chemical Name:
AXIS Axon Isolation Device, 150 μm
Synonyms
AXIS Axon Isolation Device, 150 μm
CBNumber:
CB64816892
Molecular Formula:
Molecular Weight:
0
MDL Number:
MOL File:
Mol file

AXIS Axon Isolation Device, 150 μm price

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich AX15010TC AXIS™ Axon Isolation Device, 150 µm The AXIS Axon Isolation Devices have been shown to effectively isolate cell bodies from differentiating neurites for a variety of cell types. 5UNITS $619 2022-05-15 Buy
Product number Packaging Price Buy
AX15010TC 5UNITS $619 Buy

AXIS Axon Isolation Device, 150 μm Chemical Properties,Uses,Production

General Description

AXIS Axon Isolation Devices are tissue culture ready (TC) microfluidic tools that out-of-the-box enable researchers to easily isolate, observe, and test developing neurites. They are currently being offered in four different formats to provide test flexibility during investigation.

AX15010 consists of 10 units of AX150, an axon isolation device consisting of four wells, two channels, and a set of microgrooves. Two of the wells are interconnected by a channel on each side of the device. The microgrooves are located in the area between the two channels and their length is 150μm. Each microgroove is approximately 5μm in height by 10μm in width and are designed to allow neurite passage to traverse through while preventing cell bodies from flowing across the device. There are roughly 120 microgrooves in each AX150 device.

The AXIS Axon Isolation Devices have been shown to effectively isolate cell bodies from differentiating neurites for a variety of cell types (Taylor et al., 2005; Park et al., 2006; Liu et al., 2008; Yang, et al., 2009; Taylor et al., 2009) and will likely work with most neuronal cell type that can be successfully grown in tissue culture. Because the AXIS devices are made from an inert and non-toxic polymer, cultures grown within AXIS devices can be maintained for weeks at a time. (Chun et al., 1992; Shibayama et al., 1991). In addition, because the polymer used to fabricate the axon isolation devices is optically clear, cells cultured within the device can be imaged with high resolution microscopy, including live cell imaging, confocal, and differential interference microscopy (Taylor et al., 2005; Park et al., 2006; Liu et al., 2008; Yang, et al., 2009; Taylor et al., 2009).

The microfluidic design of the AXIS devices is highly conducive to generation of hydrostatic pressure, which can be selectively used to isolate a solution, as well as chemical targets of interest, on a designated side of the device (Park et al., 2006). In addition, immunocytochemistry (IHC) can also be performed within an AXIS Axon Isolation device and a protocol to do so is provided in the manual.

AXIS Axon Isolation Device, 150 μm Preparation Products And Raw materials

Raw materials

Preparation Products

AXIS Axon Isolation Device, 150 μm Suppliers

Global( 2)Suppliers
Supplier Tel Email Country ProdList Advantage
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
Supplier Advantage
Sigma-Aldrich 80
AXIS Axon Isolation Device, 150 μm