PIPES
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|
PIPES 속성
- 녹는점
- >300 °C (lit.)
- 밀도
- 1.4983 (rough estimate)
- 굴절률
- 1.6300 (estimate)
- 저장 조건
- Sealed in dry,Room Temperature
- 용해도
- 0.1 M NaOH: 0.25 g/mL, 투명, 무색
- 물리적 상태
- 미세결정성 분말
- 색상
- 하얀색
- 산도 계수 (pKa)
- 6.8(at 25℃)
- pH 범위
- 6.1 - 7.5
- 수용성
- 물에 용해됩니다.
- 최대 파장(λmax)
- λ: 260 nm Amax: 0.055
λ: 280 nm Amax: 0.040
- Merck
- 14,7479
- BRN
- 817713
- InChIKey
- XWGRYGKFRRLUQQ-UHFFFAOYSA-N
- LogP
- -2.7 at 20℃
- CAS 데이터베이스
- 5625-37-6(CAS DataBase Reference)
안전
- 위험 및 안전 성명
- 위험 및 사전주의 사항 (GHS)
위험품 표기 | Xi | ||
---|---|---|---|
위험 카페고리 넘버 | 36/37/38 | ||
안전지침서 | 22-24/25-37/39-26 | ||
WGK 독일 | 3 | ||
TSCA | Yes | ||
HS 번호 | 29335995 |
PIPES C화학적 특성, 용도, 생산
개요
PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.화학적 성질
White/clear crystalline powder용도
A buffering agent with a pKa near physiological pH.PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] is frequently used as a buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues.Additional forms available: PIPES, Sesquisodium Salt ; PIPES dipotassium salt; PIPES disodium salt; PIPES, Sodium Salt. Buffers can be prepared by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt, titrating to the appropriate pH.
주요 응용
Glutaraldehyde fixation of plant and animal tissue samples can cause loss of lipid, leading to apparent morphological changes. Lipid loss and artifacts were minimized when PIPES was used to buffer the glutaraldehyde fixative.Alkaline phosphatase activity is lost selectively from certain rat hepatocyte organelles when fixed for ultracytochemistry with cacodylate-buffered glutaraldehyde. When PIPES was used as buffer, retention of activity was 60% greater.
Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer.
일반 설명
PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.Purification Methods
Purify PIPES from boiling water (maximum solubility is about 1g/L) or as described for ADA [N-(2-acetamido)iminodiacetic acid above]. [Good et al. Biochemistry 5 469 1966, Beilstein 23/12 V 380.]PIPES 준비 용품 및 원자재
원자재
준비 용품
PIPES 공급 업체
글로벌( 426)공급 업체
공급자 | 전화 | 이메일 | 국가 | 제품 수 | 이점 |
---|---|---|---|---|---|
Tangshan Moneide Trading Co., Ltd. | +86-315-8309571 +8615633399667 |
sales@moneidechem.com | China | 568 | 58 |
LEAP CHEM CO., LTD. | +86-852-30606658 |
market18@leapchem.com | China | 24738 | 58 |
Jiangsu Boquan Biotechnology Co., Ltd. | +86-18168774353 |
boquanshengwu003@boquansw.com | China | 248 | 58 |
Shanghai Daken Advanced Materials Co.,Ltd | +86-371-66670886 |
info@dakenam.com | China | 15928 | 58 |
Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 |
info@tianfuchem.com | China | 21691 | 55 |
Shanghai Time Chemicals CO., Ltd. | +86-021-57951555 +8617317452075 |
jack.li@time-chemicals.com | China | 1807 | 55 |
Hefei TNJ Chemical Industry Co.,Ltd. | +86-0551-65418679 +86-18949832763 |
info@tnjchem.com | China | 2989 | 55 |
career henan chemical co | +86-0371-86658258 |
sales@coreychem.com | China | 29914 | 58 |
Jinan Finer Chemical Co., Ltd | +86-531-88989536 +86-15508631887 |
sales@finerchem.com | China | 2967 | 58 |
Accela ChemBio Inc. | (+1)-858-699-3322 |
info@accelachem.com | United States | 19965 | 58 |
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