Necrostatin-1

Necrostatin-1 Basic information

Description Features In vitro

Product Name:

Necrostatin-1

Synonyms:

Necrostatin-1 - CAS 4311-88-0 - Calbiochem;IFLAB-BB F0911-6127;5-(1H-INDOL-3-YLMETHYL)-3-METHYL-2-THIOXO-4-IMIDAZOLIDINONE;5-(INDOL-3-YLMETHYL)-3-METHYL-2-THIO-HYDANTOIN;NECROSTATIN-1;MTH-DL-TRYPTOPHAN;MTH-DL-Tryptophan Methylthiohydantoin-DL-tryptophan;5-(1H-Indole-3ylmethyl)-3-methyl-2-thioxo-4-imidazolidinone

CAS:

MF:

C13H13N3OS

MW:

259.33

EINECS:

 

Product Categories:

Miscellaneous Signaling;Apoptosis Inhibitors;Inhibitors;Inhibitor

Mol File:

4311-88-0.mol

 

Necrostatin-1 Chemical Properties

Melting point 

151℃

density 

1.40±0.1 g/cm3 (20 ºC 760 Torr)

Fp 

93.3℃

storage temp. 

−20°C

solubility 

DMSO: >10 mg/mL

form 

solid

Sensitive 

Light Sensitive

 

Safety Information

Risk Statements 

36/37/39

Safety Statements 

36/37/39

WGK Germany 

3

HS Code 

29339900

MSDS Information

 

Necrostatin-1 Usage And Synthesis

Description

Necrostatin-1 is a specific RIP1 inhibitor and inhibits TNF-α-induced necroptosis with EC50 of 490 nM in 293T cells.

Features

A powerful tool for characterizing the role of necroptosis with characterized primary target.

In vitro

Necrostatin-1 (1-100 μM) inhibits the autophosphorylation of overexpressed and endogenous RIP1.It is found RIP1 is the primary cellular target responsible for the antinecroptosis activity of Necrostatin-1. Necrostatin-1 efficiently suppresses necroptotic cell death triggered by an array of stimuli in a variety of cell types. Necrostatin-1, previously identified as small-molecule inhibitor of necroptosis, inhibits RIP kinase-induced necroptosis and inhibits TNF-α-induced necroptosis in jurkat cells with EC50 of 490 nM.

Uses

Necrostatin-1 is a specific RIP1 inhibitor and inhibits TNF-α-induced necroptosis with EC50 of 490 nM.

Uses

Necroptosis is a regulated caspase-independent cell death mechanism that results in morphological features resembling necrosis. Necrostatin-1 is an inhibitor of RIP1 kinase that prevents the death of TNF-α-treated FADD-deficient Jurkat cells. Necrostatin-1 has been used to investigate the pathological importance of necroptosis in ischemic brain injury and myocardial infarction.

Biological Activity

ATP-competitive death domain receptor-associated adaptor kinase (RIP1) allosteric inhibitor (EC 50 = 182 nM). Blocks non-apoptotic cell death via inhibition of a specific cellular pathway, necroptosis, which leads to necrosis (EC 50 = 494 nM). Reduces ischemic brain injury in a mouse model of stroke.

 

Necrostatin-1 Preparation Products And Raw materials