1-Bromo-3-chloropropane:Benefits,Uses and Synthesis

1-Bromo-3-chloropropane is a colorless liquid with the formula Br(CH2)3Cl. It is used as an alkylating agent to install the –(CH2)3Cl and –(CH2)3– groups.

Benefits

1-Bromo-3-chloropropane is less toxic alternative to chloroform, it is compatible with TRI reagent for isolation of RNA with decreased DNA contamination.

Uses

1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:

feline teeth

mice brain and liver tissues

human ectocervical epithelial cells

placental samples

human mesenchymal stromal cells (hMSCs)

Synthesis

A process for the synthesis of 1-bromo-3-chloropropane by hydrobromising allyl chloride at a temperature of -10 ° C to + 50 ° C in the presence of an antimarking agent for the addition of hydrogen bromide and optionally in the presence of a solvent. The process according to claim 1, characterized in that the hydrobromination is carried out in an anhydrous liquid medium in the presence of oxygen in an amount of 0.05 to 10 wt. 2. The process according to claim 1, wherein the hydrobromination is carried out in the presence of at least one organic peroxide selected from the group consisting of dialkoylperoxide having an alkyl or chloroalkyl group having a carbon number of 1 to 12, dialkylperoxydicarbonate, bis- (chloralkyl) peroxycarbonate. acetylcyclohexanesulfonylperoxide, dibenzoylperoxide, benzoylperoxyalkyl carbonate having a carbon number of 2 to 8 in the alkyl group, di-tert. butyl peroxide and dicumyl peroxide and / or in the presence of at least one organic hydroperoxide selected from the group consisting of a target, butyl hydroperoxide, allyl chloride hydroperoxide, ethylbenzene hydroperoxide and cumene hydroperoxide in a total amount of oxygen to 0.05-10 wt.% based on allyl chloride weight.[1]

Reference

[1] CZ280614B6