| Identification | Back Directory | [Name]
Nitrotetrazolium blue chloride | [CAS]
298-83-9 | [Synonyms]
NBT
P-NBT
NBTMF
Nitro-Tb
NITRO BT
nbt(dye)
Nsc
27622
NBT Vials
NBT CHLORIDE
DITETRAZOLIUM DYE
nitrotetrazoliumbt
tetrazoliumnitrobt
p-Nitro-Blue tetra
NITROBLUETETRAZOLIN
Nitrotetrazoliumblau
tetrazoliumnitroblue
SOAP SOLUTION STABLE
Nitrotetrazolium Blu
NITRO BLUE TETRAZOLIUM
NITRO TETRAZOLIUM BLUE
P-NITROTETRAZOLIUM BLUE
P-NITRO BLUE TETRAZOLIUM
nitrobluetetrazoliumsalt
NitroBlueTetrazoliumA.R.
Nitro blue tetrazolium >99%
nitrotetrazoliumchlorideblue
p-Nitro-Blue tetrazolium chlo
NITROTETRAZOLIUM BLUE CHLORIDE
P-NitroBlueTetrazolliumChloride
NITRO BLUE TETRAZOLIUM CHLORIDE
p-Nitro-Blue tetrazolium chlori
P-NITROTETRAZOLIUM BLUE CHLORIDE
P-NITRO-BLUE TETRAZOLIUM CHLORIDE
4-NITRO BLUE TETRAZOLIUM CHLORIDE
Nbt(NitroblueTetrazoliumChloride)
NitroterazoiuM blue chlorid (NBT)
nitro blue tetrazolium dichloride
P-NITROBLUE TETRAZOLIUM DICHLORIDE
Nitrotetrazolium blue chloride(NBT)
BUFFER PHOSPHATE BUTTERFIELD (4X10)
Nitrotetrazolium Blue chloride ,90%
Nitro Blue Tetrazolium Chloride ,98%
Nitro Blue tetrazolium chloride p.a.
Nitro Blue tetrazolium chloride (NBT)
NITRO BLUE TETRAZOLIUM (NBT) REDUCTION
NITROTETRAZOLIUM BLUE CHLOR. PGE W. 10
NITRO BLUE TETRAZOLIUM CHLORIDE (NBT) FO
NBT (Nitrobluetetrazolium chloride blue)
Nitro BT (Nitro blue tetrazolium chloride)
Nitro Blue Tetrazolium chloride, tech. 90%
NITROBLUE TETRAZOLIUM CHLORIDE BIOTECH GRADE
NITRO BLUE TETRAZOLIUM GRADE III*CRYSTAL LINE
P-NITROBLUETETRAZOLIUMCHLORIDE,CRYSTAL,REAGENT
Nitro Blue Tetrazolium Chloride monohydrate,90%
Nitro Blue Tetrazolium Chloride monohydrate,85%
NITRO BLUE TETRAZOLIUMCHLORIDE ANALYTICAL GRADE
Nitro Blue Tetrazolium chloride, technical, 90%
Nitro Blue Tetrazolium [for Biochemical Research]
NITRO BLUE TETRAZOLIUM MOLECULAR*BIOLOGY REAGENT
Nitro BT~Nitrotetrazolium Blue chloride monohydrate
NITRO BLUE TETRAZOLIUM 10 MG TABLETS,*TR U-MEASURE C
P-
nitrotetrazolium Blue Nitro Blue Tetrazolium (nbt)
NITRO BLUE TETRAZOLIUM CHLORIDE MONOHYDRATE: TECH., 90%
NITRO BLUE TETRAZOLIUM CHLORIDE MONOHYDRATE extrapure AR
2,2'-bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-
2h-tetrazolium,3,3’-(3,3’-dimethoxy(1,1’-biphenyl)-4,4’-diyl)bis(2-(4-nitrophe
2h-tetrazolium,3,3’-(3,3’-dimethoxy[1,1’-biphenyl]-4,4’-diyl)bis[2-(4-nitrophe
2,20-dinitrophenyl-5,50-diphenyl-
3,30-dimethoxy-4,40-diphenylene)ditetrazolium Chloride
3,3'-(3,3'-DIMETHOXY-4,4'-BIPHENYLENE)BIS[2-(P-NITROPHENYL)-5-PHENYLTETRAZOLIUM] CHLORIDE
2,2'-DI-P-NITROPHENYL-5,5'DIPHENYL-[3,3'-DIMETHOXY-4,4'-DIPHENYLENE] DITETRAZOLIUM CHLORIDE
TETRAZOLIUM, (3,3'-DIMETHOXY-4,4'-BIPHENYLENE)BIS[2-(4-NITROPHENYL)-5-PHENYL-2H]-, CHLORIDE
3,30-(3,30-
dimethoxy-4,40-diphenylene)bis[2-(p-nitrophenyl)-5-
phenyltetrazolium Chloride]
2,2'-DI-P-NITROPHENYL-5,5'-DIPHENYL-(3,3'-DIMETHOXY)-4,4'-BISPHENYLENEDITETRAZOLIUM CHLORIDE
3,3'-(3,3'-DIMETHOXY-4,4'-BIPHENYLENE)BIS[2-(4-NITROPHENYL)-5-PHENYL-2H-TETRAZOLIUM CHLORIDE]
2-(3,3'-Dimethoxy-4'-(2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium-3-yl)-[1,1'-biphenyl]-4-yl)
3,3'-(3,3'-DIMETHOXY-4,4'-BIPHENYLENE)-BIS-(2-P-NITROPHENYL)-5-(PHENYL)-2H-TETRAZOLIUM CHLORIDE
3,3'-(3,3'-DIMETHOXY-4,4'-BIPHENYLYLENE)BIS[2-(P-NITROPHENYL)-5-PHENYL-2H-TETRAZOLIUM CHLORIDE]
3,30-(3,30-dimethoxy-4,40-biphenylylene)bis[2-(p-nitrophenyl)-
5-phenyl-2h-tetrazolium Chloride]
2,2'-Di-p-nitrophenyl-5,5'-diphenyl-(3,3'-dimethoxy)-4,4'-bisphenyleneditetrazolium Chloride
NBT
Tetrazolium, 3,30-(3,30-dimethoxy-4,40-biphenylylene)
bis[2-(p-nitrophenyl)-5-phenyl-2h-chloride]
2,2'-DI-4-NITROPHENYL-5,5'-DIPHENYL-3,3'-(3,3'-DIMETHOXY-4,4'-DIPHENYLENE)-DITETRAZOLIUM-CHLORIDE
5,5-diphenyl-3,3-bis(4-nitrophenyl)-2,2-(3,3-dimethoxybiphenyl-4,4-ylene)ditetrazolium dichloride
2,2'-BIS(4-NITROPHENYL)-5,5'-DIPHENYL-3,3'-(3,3'-DIMETHOXY-4,4'-DIPHENYLENE)DITETRAZOLIUM CHLORIDE
2H-Tetrazolium, 2,2-(3,3-dimethoxy1,1-biphenyl-4,4-diyl)bis3-(4-nitrophenyl)-5-phenyl-, dichloride
3,3'-[3,3-Dimethoxy-(1,1-biphenyl)-4,4-diyl]-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchloride]
2,20-bis(p-nitrophenyl)-
5,50-diphenyl-3,30-(3,30-dimethoxy-4,40-diphenylene)
ditetrazolium Chloride
2,20-di-p-nitrophenyl-
5,50-diphenyl-3,30-bis(3,30-dimethoxy-4,40-biphenylene)
ditetrazolium Chloride
2h-tetrazolium, 3,30-(3,30-dimethoxy-
4,40-biphenylylene)bis[2-(p-nitrophenyl)-5-phenyl]-,
dichloride
2,
20-bis(p-nitrophenyl)-5,50-diphenyl-3,30-(3,30-dimethoxy-
4,40-biphenylylene)ditetrazolium Chloride
5,5'-diphenyl-3,3'-bis(4-nitrophenyl)-2,2'-(3,3'-dimethoxybiphenyl-4,4'-ylene)ditetrazolium dichloride
3,3'-(3,3'-DIMETHOXY[1,1'-BIPHENYL]-4,4'-DIYL)BIS[2-(4-NITROPHENYL)]-5-PHENYL-2H-TETRAZOLIUM DICHLORIDE
3,3'-(3,3'-Dimethoxy-[1,1'-biphenyl]-4,4'-diyl)bis(2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium) chloride
2H-TETRAZOLIUM,2,2'-((3,3')-DIMETHOXY[1,1'-BIPHENYL]-4,4'-DIYL)BIS[3-(4-NITROPHENYL)-5-PHENYL-],DICHLORIDE
2H-Tetrazolium, 3,3'-(3,3'-dimethoxy[1,1'- biphenyl]-4,4'-diyl)bis[2-(4-nitrophenyl)-5-phenyl -, dichloride
2h-tetrazolium, 2,20-(3,30-dimethoxy
[1,10-biphenyl]-4,40-diyl)bis[3-(4-nitrophenyl)-5-phenyl]-,
dichloride
2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, chloride (1:2)
Nitrotetrazolium blue chloride
2,2'-Bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride
Nitrotetrazolium Blue chloride,2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-
p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT, 3,3μ-(3,3μ-Dimethoxy-4,4μ-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,2μ-bis(4-Nitrophenyl)-5,5μ-diphenyl-3,3μ-(3,3μ-dimethoxy-4,4μ-diphenylene)ditetrazolium chloride
p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT, Nitrotetrazolium Blue chloride, 3,3μ-(3,3μ-Dimethoxy-4,4μ-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,2μ-bis(4-Nitrophenyl)-5,5μ-diphenyl-3,3μ-(3,3μ-dimethoxy-4,4μ-diphenylene)ditetrazolium chloride | [EINECS(EC#)]
206-067-4 | [Molecular Formula]
C40H30Cl2N10O6 | [MDL Number]
MFCD00012159 | [MOL File]
298-83-9.mol | [Molecular Weight]
817.64 |
| Chemical Properties | Back Directory | [Appearance]
yellow crystals | [Melting point ]
200°C | [density ]
1.5521 (rough estimate) | [refractive index ]
1.7350 (estimate) | [storage temp. ]
2-8°C
| [solubility ]
methanol: 50 mg/mL, clear, very strongly yellow
| [form ]
Powder/Solid | [color ]
Yellow | [Stability:]
Stable, but store cold. | [Water Solubility ]
Soluble in water, ethanol and methanol. | [Sensitive ]
Light Sensitive | [λmax]
256 nm | [BRN ]
4115923 | [Biological Applications]
Diagnosis of Alzheimer’s disease,bacterial vaginosis,behavioral disturbances in children,Hirschsprung disease; phosphatase assay; phytase assay; detecting bacteria,microorganisms,phosphoinositides,yeast; generating and detecting | [EPA Substance Registry System]
2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[ 1,1'-biphenyl]-4,4'- diyl)bis[3-(4-nitrophenyl)- 5-phenyl-, dichloride(298-83-9) |
| Hazard Information | Back Directory | [Description]
Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which results in the production of blue reduced NBT formazan in normal cells but not in those from patients with chronic granulomatous disease. NBT can also be used as a chromogenic activity stain for oxidoreductases in gels or solutions. More commonly NBT is often paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) for the colorimetric detection of alkaline phosphatase activity. Alkaline phosphate converts PCIB to a product that reduces NBT to its formazan derivative, resulting in a black-purple precipitate. | [Chemical Properties]
NBT is a yellow crystals that can soluble in water, aqueous buffers, and dimethylformamide. Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology. | [Uses]
Nitrotetrazolium Blue Chloride is a Tetrazolium dye used in colorimetric assays. It can be used to assess cell viability as a function of redox potential. Intense blue dye used in histochemistry determinations. actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate. | [Application]
Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.
It has been used as a substrate for western blot detection.
It has been used for the staining of histological tissue sections.
Nitro Blue Tetrazolium chloride is used to confirm diagnosis of chronic granulomatous disease.
It is a microscopic stain, which is used to detect dehydrogenases and alkaline phosphatase. It acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase.
Further, it is used in immunology for detection of alkaline phosphatase. | [Definition]
ChEBI: Nitro blue tetrazolium dichloride is an organic chloride salt having nitro blue tetrazolium(2+) as the counterion. It contains a nitro blue tetrazolium(2+). | [General Description]
Nitrotetrazolium Blue chloride (NBT) is a colorless to yellow dye that is reducible to blue or black formazan crystals by certain cells; formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease. | [Hazard]
Moderately toxic by ingestion. | [Biochem/physiol Actions]
Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target. | [Safety Profile]
Moderately toxic by ingestion. When heated to decomposition it emits toxic vapors of NOx and Clí. | [storage]
+4°C |
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