| Identification | More | [Name]
Hoechst 33258 | [CAS]
23491-45-4 | [Synonyms]
2-[2-(4-HYDROXYPHENYL)-6-BENZIMIDAZOLYL]-6-(1-METHYL-4-PIPERAZYL)-BENZIMIDAZOLE TRIHYDROCHLORIDE
2-[-2-(4-HYDROXYPHENYL)-6-BENZIMIDAZOYL]-6-(1-METHYL-4-PIPERAZYL)-BENZIMIDAZOLE, 3HCL
2-[2-(4-HYDROXYPHENYL)-6-BENZIMIDAZOYL]-6-(1-METHYL-4-PIPERAZYL)BENZIMIDAZOLE TRIHYDROCHLORIDE
2'-(4-HYDROXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5'-BI(1H-BENZIMIDAZOLE) TRIHYDROCHLORIDE
BISBENZIMIDE
BISBENZIMIDE H 33258
BISBENZIMIDE H 33258 FLUOROCHROME, TRIHYDROCHLORIDE
BISBENZIMIDE H 33258 TRIHYDROCHLORIDE
BISBENZIMIDE TRIHYDROCHLORIDE
H33258
HOE 33258
HOECHST 33258
HOECHST NO 33258
HOECHST STAIN SOLUTION
33258hoechst
4-(5-(4-methyl-1-piperazinyl)(2,5’-bi-1h-benzimidazol)-2’-yl)-phenotrihy
4-[5-(4-methyl-1-piperazinyl)[2,5’-bi-1h-benzimidazol]-2’-yl]-phenotrihydr
bisbenzimidazoletrihydrochloride
bisbenzimidehydrochloride
hoe33058 | [EINECS(EC#)]
245-690-6 | [Molecular Formula]
C25H27Cl3N6O | [MDL Number]
MFCD00012679 | [Molecular Weight]
533.88 | [MOL File]
23491-45-4.mol |
| Chemical Properties | Back Directory | [Appearance]
yellow to yellow-green powder | [Melting point ]
314 °C | [density ]
1.5099 (rough estimate) | [refractive index ]
1.6300 (estimate) | [storage temp. ]
2-8°C
| [solubility ]
H2O: 10 mg/mL
| [form ]
powder
| [color ]
Yellow to Very Dark Yellow | [Water Solubility ]
SOLUBLE | [BRN ]
4088183 | [Stability:]
Hygroscopic | [InChIKey]
UDJDXLXTHZQAAC-UHFFFAOYSA-N | [SMILES]
CN1CCN(C2C=CC3NC(C4C=CC5NC(C6C=CC(O)=CC=6)=NC=5C=4)=NC=3C=2)CC1.Cl | [CAS DataBase Reference]
23491-45-4(CAS DataBase Reference) | [EPA Substance Registry System]
23491-45-4(EPA Substance) |
| Safety Data | Back Directory | [Hazard Codes ]
Xn | [Risk Statements ]
R22:Harmful if swallowed.
R36/38:Irritating to eyes and skin .
R36/37/38:Irritating to eyes, respiratory system and skin .
R20/21/22:Harmful by inhalation, in contact with skin and if swallowed . | [Safety Statements ]
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S36:Wear suitable protective clothing .
S24/25:Avoid contact with skin and eyes .
S23:Do not breathe gas/fumes/vapor/spray (appropriate wording to be specified by the manufacturer) . | [WGK Germany ]
3
| [RTECS ]
SM1140500
| [F ]
3-8-10 | [TSCA ]
TSCA listed | [HS Code ]
32129000 | [Storage Class]
11 - Combustible Solids | [Toxicity]
dog,LD,intravenous,> 22400ug/kg (22.4mg/kg),LUNGS, THORAX, OR RESPIRATION: DYSPNEASENSE ORGANS AND SPECIAL SENSES: LACRIMATION: EYEGASTROINTESTINAL: CHANGES IN STRUCTURE OR FUNCTION OF SALIVARY GLANDS,National Technical Information Service. Vol. PB84-171206, |
| Hazard Information | Back Directory | [Chemical Properties]
yellow to yellow-green powder | [Uses]
Hoechst 33258 is useful for staining DNA, chromosomes and nuclei. The nucleic acid binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence
| [General Description]
Membrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. | [Biochem/physiol Actions]
bisBenzimide H 33258 is a potent and selective Bcl-XL inhibitor. bisBenzimide H 33258 is a membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA. | [storage]
+4°C (desiccate) |
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