| Identification | Back Directory | [Name]
TOLUIDINE BLUE FOR MICROSCOPY | [CAS]
6586-04-5 | [Synonyms]
Toluidine bule, BS
TOLUIDINE BLUE C.I. No 52040
TOLUIDINE BLUE FOR MICROSCOPY
Toluidine Blue, for microscopy (Hist., Vit.)
(7-amino-8-methylphenothiazin-3-ylidene)-dimethylazanium,dichlorozinc,dichloride
Zinc chloride compd. with 3-amino-7-(dimethylamino)-2-methylphenothiazin-5-ium chloride
Phenothiazin-5-ium, 3-amino-7-(dimethylamino)-2-methyl-, chloride, compd. with zinc chloride (ZnCl2) | [EINECS(EC#)]
202-146-2 | [Molecular Formula]
C15Cl1H16N3S1 | [MDL Number]
MFCD00011934 | [MOL File]
6586-04-5.mol | [Molecular Weight]
373.97 |
| Chemical Properties | Back Directory | [storage temp. ]
room temp | [solubility ]
methanol: 0.01g/10 mL, blue to very deep blue | [Colour Index ]
52040 | [form ]
powder or crystals | [color ]
green to very dark green | [BRN ]
4068052 | [InChI]
InChI=1S/C15H16N3S.3ClH.Zn/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;;;;/h4-8H,16H2,1-3H3;3*1H;/q+1;;;;+2/p-3 | [InChIKey]
MOZNQPRVXVTENQ-UHFFFAOYSA-K | [SMILES]
N(C1C=CC2=NC3=CC(C)=C(N)C=C3[S+]=C2C=1)(C)C.[Zn](Cl)Cl.[Cl-] |
| Hazard Information | Back Directory | [Uses]
Toluidine Blue is suitable for use in the following applications:- to demonstrate amyloid; DNA in plant material, mast cells, Nissl substance, nuclei, and sulfatides
- in metachromatic staining of sulfated mucins and related materials
- as staining agent to identify the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone.
- staining of mucins in water-miscible resins and of nucleic acids in epoxy resins(1)
| [Preparation]
Toluidine Blue O 47[6586-04-5] is prepared from N,N-dimethyl-p-phenylenediamine and o-toluidine and is used as a stain for microscopy.
| [Biological Activity]
Toluidine Blue is a basic thiazine metachromatic dyewhich is used for selectively staining acidic tissue components such as carboxylatessulfatesand phosphate radicals.It is found to have an affinity for nucleic acids and therefore binds to the nuclear material of tissues with a high DNA and RNA content. |
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