| Identification | More | [Name]
Direct Blue 14 | [CAS]
72-57-1 | [Synonyms]
3,3'-DIMETHYL-4,4'-(BIPHENYLENEBISAZO)BIS(8-AMINO-1-NAPHTHOL)-3,6-DISULFONIC ACID TETRASODIUM SALT
4,4'-BIS(8-AMINO-3,6-DISULFO-1-HYDROXY-2-NAPHTHYLAZO)-3,3'-DIMETHYLBIPHENYL TETRASODIUM SALT
BENZO BLUE 3B
CHROME LEATHER BLUE 3B
CI 23850
CI NO 23850
CONGO BLUE 3B
DIAMINE BLUE 3B
DIRECT BLUE 14
DIRECT BLUE 3B
NIAGARA BLUE 3B
PONTAMINE BLUE 3BX
TRUE BLUE
TRYPAN BLUE
TRYPAN BLUE BPC
2,7-naphthalenedisulfonicacid,3,3’-((3,3’-dimethyl-4,4’-biphenylylene)bis(azo
2,7-naphthalenedisulfonicacid,3,3’-[(3,3’-dimethyl[1,1’-biphenyl]-4,4’-
2,7-Naphthalenedisulfonicacid,3,3’-[[3,3’-dimethyl(1,1’-biphenyl)-4,4’-diyl]bis(azo)]bis(5-amino-4-hydroxy-,tetrasodiumsalt
amanilskybluer
amidineblue4b | [EINECS(EC#)]
200-786-7 | [Molecular Formula]
C34H24N6Na4O14S4 | [MDL Number]
MFCD00003969 | [Molecular Weight]
960.81 | [MOL File]
72-57-1.mol |
| Chemical Properties | Back Directory | [Appearance]
Trypan Blue is a dark Blue crystalline solid or
powder. | [Melting point ]
>300 °C (lit.) | [density ]
1.007 g/mL at 20 °C
| [storage temp. ]
Store at RT. | [solubility ]
H2O: soluble10mg/mL | [Colour Index ]
23850 | [form ]
Powder | [color ]
Dark greenish-brown | [PH]
9.8 (10g/l, H2O, 20℃) | [Stability:]
Stable. Incompatible with strong oxidizing agents. | [Water Solubility ]
10 g/L (25 ºC) | [Merck ]
14,9792 | [BRN ]
4360496 | [CAS DataBase Reference]
72-57-1(CAS DataBase Reference) | [IARC]
2B (Vol. 8, Sup 7) 1987 | [EPA Substance Registry System]
72-57-1(EPA Substance) |
| Safety Data | Back Directory | [Hazard Codes ]
T,Xi | [Risk Statements ]
R45:May cause cancer.
R41:Risk of serious damage to eyes.
R37/38:Irritating to respiratory system and skin .
R36/37/38:Irritating to eyes, respiratory system and skin .
R22:Harmful if swallowed. | [Safety Statements ]
S53:Avoid exposure-obtain special instruction before use .
S45:In case of accident or if you feel unwell, seek medical advice immediately (show label where possible) .
S36/37/39:Wear suitable protective clothing, gloves and eye/face protection .
S36:Wear suitable protective clothing .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . | [RIDADR ]
2811 | [WGK Germany ]
3
| [RTECS ]
QJ6475000
| [TSCA ]
Yes | [HS Code ]
32041400 | [Safety Profile]
Suspected carcinogen
with experimental carcinogenic,
neoplastigenic, and tumorigenic data. Poison
by intraperitoneal, intravenous, and
subcutaneous routes. Experimental
teratogenic and reproductive effects.
Mutation data reported. When heated to
decomposition it emits very toxic fumes of
NOx NazO, and SOx | [Hazardous Substances Data]
72-57-1(Hazardous Substances Data) | [Toxicity]
LD100 i.v. in rats: 300 mg/kg (Anderson) |
| Hazard Information | Back Directory | [General Description]
Bluish-gray to dark blue powder. | [Reactivity Profile]
TRYPAN BLUE(72-57-1) is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. This chemical is incompatible with strong oxidizing agents and strong acids. | [Air & Water Reactions]
Azo dyes can be explosive when suspended in air at specific concentrations. Slightly soluble in water. | [Health Hazard]
ACUTE/CHRONIC HAZARDS: This chemical may cause irritation and may be absorbed through the skin. It is a positive animal carcinogen. When heated to decomposition this compound emits very toxic fumes of nitrogen oxides, sulfur oxides, carbon monoxide and carbon dioxide. | [Potential Exposure]
Used in dyeing textiles; leather and
paper; as a biological stain. | [Fire Hazard]
Flash point data for this chemical are not available; however, TRYPAN BLUE is probably combustible. | [First aid]
If this chemical gets into the eyes, remove any
contact lenses at once and irrigate immediately for at least
15 minutes, occasionally lifting upper and lower lids. Seek
medical attention immediately. If this chemical contacts the
skin, remove contaminated clothing and wash immediately
with soap and water. Seek medical attention immediately.
If this chemical has been inhaled, remove from exposure,
begin rescue breathing (using universal precautions, including
resuscitation mask) if breathing has stopped and CPR if
heart action has stopped. Transfer promptly to a medical
facility. When this chemical has been swallowed, get medical
attention. Give large quantities of water and induce
vomiting. Do not make an unconscious person vomit. | [Shipping]
UN2811 Toxic solids, organic, n.o.s., Hazard
Class: 6.1; Labels: 6.1-Poisonous materials, Technical
Name Required. UN3143 dyes, solid, toxic, n.o.s. or dye
intermediates, solid, toxic, n.o.s., Hazard Class: 6.1;
Labels: 6.1-Poisonous materials, Technical Name Required. | [Incompatibilities]
Dust may form explosive mixture with
air. Trypan Blue is incompatible with strong oxidizing
agents. Incompatible with oxidizers (chlorates, nitrates,
peroxides, permanganates, perchlorates, chlorine, bromine,
fluorine, etc.); contact may cause fires or explosions. Keep
away from alkaline materials, strong bases, strong acids,
oxoacids, epoxides. Azo compounds can detonate. This
applies in particular to organic azides that have been sensitized
by the addition of metal salts or strong acids. Toxic
gases are formed by mixing materials of this class with
acids, aldehydes, amides, carbamates, cyanides, inorganic
fluorides, halogenated organics, isocyanates, ketones,
metals, nitrides, peroxides, phenols, epoxides, acylhalides,
and strong oxidizing or reducing agents. Flammable gases
are formed by mixing materials in this group with alkali
metals. Explosive combinations can occur with strong oxidizing
agents, metal salts, peroxides, and sulfides. Trypan
Blue is sensitive to prolonged exposure to heat. | [Chemical Properties]
blueish grey powder | [Chemical Properties]
Trypan Blue is a dark Blue crystalline solid or
powder. | [Waste Disposal]
Consult with environmental
regulatory agencies for guidance on acceptable disposal
practices. Generators of waste containing this contaminant
(≥100 kg/mo) must conform with EPA regulations governing
storage, transportation, treatment, and waste
disposal. | [Uses]
Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells. | [Uses]
Biological stain. | [Uses]
Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples. Dyes and metabolites. | [Definition]
ChEBI: An organosulfonate salt that is the tetrasodium salt of 3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis(5-amino-4-hydroxynaphthalene-2,7-disulfonic acid). | [Preparation]
3,3′-Dimethylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-2,7-disulfonic acid(2 Moore) coupled. | [Biochem/physiol Actions]
Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells. | [Staining Procedures]
A 0.01-0.2% aqueous solution is used for root staining. The method is often used for investigation of mycorrhizas. Suitable for whole mounts as well as sections. Also used as a stain for vitality, as living cells generally remain unstained. | [Properties and Applications]
blue. Blue gray powder. Soluble in water for blue, slightly soluble in soluble fiber element, insoluble in other organic solvents. The strong sulfuric acid in dark green light for blue, diluted into a red light blue; In the light of nitric acid for palm gray solution. The dye solution to join a red sulfuric acid 10% change; Add thick sodium hydroxide solution for red light purple.
|
Standard
|
Acid Resistance
|
Alkali Resistance
|
Light Fastness
|
Soaping
|
Water
|
|
Fading
|
Stain
|
Fading
|
Stain
|
|
ISO
|
4-5
|
4
|
1-2
|
2
|
|
2
|
|
|
AATCC
|
5
|
2
|
1-2
|
2
|
|
1
|
|
|
|
|